Abstract:Objective To build a stable cell line with S100A4 high expression and study the relationship between S100A4 and metastasis and invasion of nasopharyngeal carcinoma (NPC). Methods To amplify the fragment of S100A4 by PCR method, and PCR products were inserted into pCMV vector. The reconstructed pCMV-S100A4 vector and empty vector were transferred into CNE2 cell line. Then the growth curve were drown by MTT method. The expression of S100A4, E-cadherin, Fibronectin, MMP2 (matrix metallopeptidase 2) and MMP9 (matrix metallopeptidase 9) were detected by Western blot. And the changes of ability of metastasis and invasion were examined by Transwell. Results Succeed to build a stable cell line with S100A4 high expression; The grow curves showed that the growth speed were more quickly in pCMV-S100A4-CNE2 than in pCMV-empty-CNE2 and CNE2. Western blot results showed that there were low expression of E-cadherin、Fibronectin and high expression of MMP2, MMP9 in pCMV-S100A4-CNE2 than in pCMV-empty-CNE2 and CNE2. Transwell results showed that there were more invaded cells in pCMV-S100A4-CNE2 than in pCMV-empty-CNE2 and CNE2 (P < 0.01). Conclusions S100A4 can regulate the process of EMT in NPC and upregulate the expression of MMP2 and MMP9 to promote the metastasis and invasion of NPC cells.