多次吸入七氟烷对新生期不同性别大鼠行为学的影响

Repeated Sevoflurane inhalation in neonatal period on learning and memory of rats with different genders

摘要:

目的 探讨新生期间断重复吸入七氟烷对不同时期和性别大鼠学习记忆能力的影响。方法 选 取48 只新生SD 大鼠，分为七氟烷雄性组、七氟烷雌性组，以及对照雄性组、对照雌性组，每组12 只大鼠。 七氟烷组大鼠在出生后第7、14、21 天分别吸入2.6% 七氟烷+ 运载气体（1 L/min 空气+1 L/min 氧气） 2 h。对照组在相同时间点吸入运载气体，自然喂养至幼年期（31 ～ 37 d）行Morris 水迷宫实验，观察定位航 行实验游泳路程、逃避潜伏时间及空间探索实验各指标；成年期（91 ～ 97 d）行定位航行和空间探索实验， 观察指标同前。结果 ①幼年期大鼠游泳路程多于成年期大鼠；②幼年期雌性大鼠定位航行和空间探索实验 各指标与雄性大鼠比较，差异无统计学意义（P >0.05）；③成年期雌性大鼠定位航行和空间探索实验各指标 与雄性大鼠比较，差异无统计学意义（P >0.05）。结论 大鼠幼年期学习记忆能力可能产生一过性轻度损害， 但空间参考记忆能力无明显改变；而成年期均无明显影响；新生期间断重复七氟烷吸入麻醉后，不同性别大 鼠学习记忆能力无明显差别。

Abstract:

Objective To explore the impact of neonatal-period repeated inhalation of Sevoflurane on learning and memory of rats with different genders and in different periods. Methods Forty-eight newborn SD rats were divided into Sevoflurane male group (Sev M group, n = 12), Sevoflurane female group (Sev F group, n = 12), control male group (Con M group, n = 12) and control female group (Con F group, n = 12). The rats of the Sev M group and the Sev F group inhaled 2.6% Sevoflurane and carrier gas (1 L/min air + 1 L/min oxygen) for 2 h on the 7th, 14th and 21st d after birth; while the rats in the control groups inhaled only carrier gas for 2 h on the same days. The rats were naturally fed till infancy (31-37 d), and Morris water maze experiment was done, swimming distance and escape latency time ibatch feeding rats to adulthood (91-97 d), navigation and space exploration experiments were done again, the same indexes were observed. Results The total swimming distance of the infant rats was longer than that of the adult rats. The indexes of the navigation and space exploration experiments were not significantly different between the female and male infant rats (P > 0.05). There was no significant difference in any index of the positioning navigation and space exploration experiments between the adult female and male rats (P > 0.05). Conclusions Repeated inhalation of Sevoflurane may cause temporary and mild damage of learning and memory ability without change in spatial reference memory in infant rats; however it has no impact on adult rats. After repeating Sevoflurane anesthesia there is no obvious difference in learning or memory ability between newborn rats of different genders.n the navigation experiment and the indexes in the space exploration experiment were observed. With