TIM-1 和TIM-4 mRNA 在HAV 患者中的表达及其临床意义

Clinical significance of TIM-1 and TIM-4 mRNA levels in patients with hepatitis A

摘要:

目的 观察典型病程甲型病毒性肝炎（HAV）患者外周血中T 细胞免疫球蛋白域蛋白-1 （TIM-1）和T 细胞免疫球蛋白域蛋白-4（TIM-4）mRNA 的表达水平，探讨HAV 患者病情进展的不同时 期TIM-1 和TIM-4 mRNA 水平与HAV RNA 含量，HAV 免疫球蛋白M（IgM）、免疫球蛋白G（IgG）浓度 及肝功能指标的关系。方法 选取HAV 典型病程的30 例患者作为HAV 组，分别于潜伏期或黄疸前期、黄 疸期及恢复期留取血液标本，以同期30 例健康体检者作为对照组，收集血液标本。采用实时荧光定量聚合 酶链反应检测TIM-1 和TIM-4 mRNA 表达水平，同时检测HAV 患者外周血HAV RNA 含量，酶联免疫 吸附法检测HAV 患者外周血HAV 抗体IgM、IgG 滴度水平，全自动生化分析仪检测肝功能指标谷丙转氨 酶、谷草转氨酶及总胆红素。结果 HAV 组患者外周血TIM-1 和TIM-4 mRNA 表达水平高于对照组患者 （P <0.05）。随着疾病进程的继续，TIM-1 及TIM-4 mRNA 表达水平于黄疸期达峰值，恢复期下降。TIM- 1 mRNA 表达水平与HAV 患者IgM 滴度、AST 及TBIL 呈正相关（P <0.05），与IgG 滴度无关（P >0.05）。 结论 HAV 患者外周血中TIM-1 和TIM-4 mRNA 的表达水平与HAV 的疾病进展有较好的拟合性，TIM-1 和TIM-4 mRNA 可能在HAV 的发生、发展中发挥作用。

Abstract:

Objective To observe the expression levels of T cell immunoglobulin domain and mucin domain 1 (TIM-1) and TIM-4 mRNAs in peripheral blood of the patients with typical courses of viral hepatitis A, and to investigate the relationships of TIM-1 and TIM-4 mRNAs with RNA content of HAV, concentrations of anti-HAV IgM and IgG, and parameters of hepatic function. Methods Blood specimens were taken from 30 patients with typical courses of viral hepatitis A in incubation or pre-icterus stage, icterus stage and recovery stage and 30 healthy controls. TIM-1 and TIM-4 mRNA levels in the peripheral blood were assayed by RT-PCR. Also the HAV RNA content in the peripheral blood was detected. The concentrations of HAV antibody IgM and IgG in the peripheral blood of the viral hepatitis A patients were assayed by ELISA. The parameters of hepatic function (ALT, T-bil and D-bil) were assayed by the Hitachi 7600 Auto-biochemical Analyzer. Results The expression levels of TIM-1 and TIM-4 mRNAs in the peripheral blood of the hepatitis A patients were dramatically higher than those of the control group (P < 0.05), reached the peak value at the icterus stage and declined during the recovery stage. The expression levels of TIM-1 and TIM-4 mRNAs were in positive correlations with the titer of anti-HAV IgM and the parameters of hepatic function in the hepatitis A patients (P < 0.05), but did not have correlation with the titer of anti-HAV IgG (P > 0.05). Conclusions Expression levels of TIM-1 and TIM-4 mRNAs in the peripheral blood of hepatitis A patients have high degree to match the development of hepatitis A. TIM-1 and TIM-4 mRNAs are likely related to the incidence and development of hepatitis A.