氨基酸转运蛋白家族成员在膀胱癌中的表达及其临床意义

Expressions of amino acid transporters in bladder cancer tissues and their clinical significance

摘要:

目的 探讨氨基酸转运蛋白家族成员在膀胱癌中的表达及意义。方法 免疫组织化学法检测93 例 膀胱癌患者癌标本中L 型氨基酸转运载体1（LAT1）、系统ASC 氨基酸转运蛋白2（ASCT2）、胱氨酸/ 谷 氨酸转运体功能亚基（xCT）、Ki-67 和CD34 的表达。结果 LAT1、ASCT2 和xCT 的阳性表达率分别为 55.9%（52/93）、77.4%（72/93）和65.6%（61/93）。ASCT2 的阳性表达与LAT1、Ki-67 相关。单因素分析表 明，ASCT2 表达、疾病分期与总生存期关系密切。而肿瘤直径、疾病分期与无病生存期关系密切。多元回归 分析证实，ASCT2 表达是膀胱癌患者术后预后差的独立预测因子。结论 ASCT2 能够作为膀胱癌患者的预 后判断指标。

Abstract:

Objective To investigate the clinicopathological significance of the amino acid transporters in patients with bladder cancer. Methods The surgically resected bladder cancer tissues of 93 patients were stained by immunohistochemistry for detection of the expressions of L-type amino acid transporter 1 (LAT1), system ASC amino acid transporter 2 (ASCT2), x-C-type cystine/glutamate transporter (xCT), Ki-67 and CD34. Results The positive expresseion rates of LAT1, ASCT2 and xCT were 55.9% (52/93), 77.4% (72/93) and 65.6% (61/93), respectively. Positive ASCT2 expression was significantly associated with LAT1 and Ki-67 expressions. By univariate analysis, ASCT2 expression and disease stage had close relationships with overall survival. Tumor size and disease stage had close relationships with disease-free survival. Multivariate analysis confirmed that ASCT2 expression was an independent predictor for poor outcome after surgery. Conclusions ASCT2 can serve as a significant marker for prediction of negative prognosis after surgery.