三七护肝胶囊对小鼠酒精性肝损伤的保护作用

Study on protective effect of Sanqi Hugan Capsules against alcoholic liver injury in mice

摘要:

目的 探讨三七护肝胶囊对50% 乙醇所致小鼠酒精性肝损伤的保护作用。方法 SPF 级昆明种 雄性小鼠按体重随机分为空白对照组、模型对照组，以及三七护肝胶囊高、中、低剂量组，每组10 只。连续 灌胃30 d 后，除空白对照组外，其余4 组灌胃50% 乙醇复制小鼠急性酒精性肝损伤模型。模型复制后16 h 检 测小鼠血清中谷丙转氨酶(ALT）、谷草转氨酶（AST）含量，以及肝匀浆中丙二醛（MDA）、超氧化物歧化 酶（SOD）、谷胱甘肽（GSH）、三酰甘油（TG）含量，并观察肝脏病理组织形态学变化。结果 三七护肝胶 囊高、中剂量组可降低小鼠血清ALT、AST 和肝匀浆中MDA、TG 含量，三七护肝胶囊高剂量组可提高肝匀 浆中SOD、GSH 活性。肝脏病理组织形态学结果显示，三七护肝胶囊高剂量组小鼠肝细胞脂肪变性程度较轻。 结论 三七护肝胶囊对酒精性肝损伤具有保护作用。

Abstract:

Objective To study the protective effect of Sanqi Hugan Capsules (SHC) against alcoholic liver injury in mice induced by 50% ethanol. Methods SPF-class Kunming male mice were randomly divided into 5 groups, i.e. normal control group, model control group, high-, medium- and low-dosage SHC groups with 10 in each group. After 30 days of continuous gavage, injuried liver models were made with 50% ethanol orally except the normal control group. After 16 h, the serum content of ALT and AST and the levels of MDA, SOD, GSH and TG in liver homogenate were determined, the histopathologic changes of the hepatic tissues were observed. Results The serum concentrations of ALT and AST, and the liver homogenate levels of MDA and TG in the middle-dosage and high-dosage groups were significantly decreased, and the SOD and GSH levels in the liver homogenate of the highdosage group were significantly increased. Furthermore histopathological examination revealed fatty degeneration of the hepatic cells was significantly decreased in the high-dosage group. Conclusions SHC could protect liver against alcoholic injury.