ZNF267 在膀胱癌中的表达及其对细胞增殖和转移的影响

Expression of ZNF267 in bladder cancer and its effect on proliferation and migration of TCC cells

摘要:

目的 研究锌指蛋白267（ZNF267）在膀胱移行细胞癌（TCC）组织及细胞中的表达，及其 对TCC 细胞增殖、凋亡、周期、转移及侵袭的影响。方法 通过实时荧光定量聚合酶链反应检测TCC 组 织及细胞中ZNF267 的表达，并利用siRNA 沉默TCC 中ZNF267 的表达。采用CCK-8 法检测ZNF267 对 TCC 细胞增殖的影响，流式细胞术检测ZNF267 对TCC 细胞凋亡及周期的影响，Transwell 实验检测ZNF267 对TCC 细胞转移和侵袭的影响。结果 TCC 组织和细胞中ZNF267 的表达水平高于癌旁组织和膀胱上皮 永生化细胞系。沉默TCC 细胞中ZNF267 的表达后，细胞的增殖能力受抑制，而凋亡能力增强。此外，与 ZNF267-NC 组细胞相比，转染ZNF267 siRNA 的TCC 细胞周期被阻滞于G1 期，细胞转移和侵袭能力受抑制。 结论 ZNF267 在TCC 中表达上调，并可促进TCC 细胞的增殖和转移。

Abstract:

Objective To investigate the expression of zinc finger 267 (ZNF267) in transitional cell carcinoma (TCC), and its effect on proliferation, apoptosis, cell cycle, migration and invasion of TCC cells. Methods qRTPCR was performed to detect the expression of ZNF267 in tissues and cells of TCC. ZNF267 expression of TCC cells was silenced by siRNA. After silencing the expression of ZNF267 in TCC cells, the cell proliferation was detected by CCK-8, cell apoptosis and cell cycle were detected by flow cytometry. Furthermore, the effect of ZNF267 on cell migration and cell invasion was detected by Transwell assay. Results The expression of ZNF267 was up-regulated in the TCC tissues and cells compared with the adjacent tissues and immortalized human bladder urothelial cells. Compared with the ZNF267-normal TCC cells, the proliferation of the TCC cells transfected with ZNF267 siRNA was significantly inhibited, but the cell apoptosis ability was significantly promoted. Furthermore, the cell cycle of the TCC cells transfected with ZNF267 siRNA was arrest at G1 stage, the cell migration and invasion were decreased in the TCC cells transfected with ZNF267 siRNA. Conclusions ZNF267 is significantly upregulated in TCC tissues and cells, and could promote the proliferation and migration of TCC cells.