Vav1 蛋白在胃癌中的表达及其临床意义

Significance of Vav1 protein detection in peripheral blood of patients with gastric cancer

摘要:

目的 检测Vav1 蛋白在胃癌患者外周血和肿瘤组织中的表达，并对其相关性和临床意义进行探 讨。方法 选取行胃癌根治术的200 例患者作为手术组，同期就诊的术后复发患者62 例作为复发组，年龄、 性别相匹配的健康体检者100 例作为对照组。取肿瘤组织及正常胃黏膜组织的石蜡标本进行免疫组织化学SP 法染色，检测肿瘤组织及周围正常黏膜组织中Vav1 蛋白的表达；酶联免疫吸附试验检测各组外周血中Vav1 蛋白的含量。结果 手术组患者术前外周血中Vav1 浓度高于对照组（P <0.05）；术后Vav1 浓度下降，与对 照组比较差异无统计学意义（P >0.05）。复发组外周血Vav1 水平高于手术组术前水平（P <0.05）。手术组肿 瘤组织Vav1 蛋白表达较正常胃黏膜升高（P <0.05）。复发组肿瘤组织Vav1 蛋白表达较手术组升高（P <0.05）。 手术组和复发组患者肿瘤组织与外周血Vav1 蛋白表达有相关性（P <0.05）。结论 Vav1 蛋白在胃癌患者外 周血及肿瘤组织中表达升高，且有相关性。外周血Vav1 可作为新的肿瘤标志物，对评价肿瘤变化、预测肿瘤 复发有重要意义。

Abstract:

Objective To explore the expression of Vav1 protein in peripheral blood and gastric cancer tissues and its relationship and clinical significance. Methods In this study, 200 patients with pathological diagnosis of gastric cancer who accepted radical surgery were recruited as operation group, and 62 patients diagnosed as recrudescence after operation were recruited as recurrent group, also 100 normal cases were recruited as control group. Immunohistochemical technique was performed to test the expression of Vav1 protein in the paraffin sections of gastric cancer tissues and normal mucosa, and the expression of Vav1 protein in peripheral blood was tested with enzyme-linked immunosorbent assay (ELISA). Results The level of Vav1 protein in the peripheral blood of the operation group before operation was higher than that of the control group (P < 0.05). After operation, the level of Vav1 in the peripheral blood of the operation group decreased significantly, which was not significantly different from that of the control group (P > 0.05). The expression of Vav1 protein in the peripheral blood of the recurrent group was higher than the preoperative level of the operation group (P < 0.05). The expression of Vav1 protein in the gastric cancer tissues of the operation group was higher than that in the normal gastric mucosa (P < 0.05). The expression of Vav1 protein in the recurrent group was higher than that in the operation group (P < 0.05). Significant correlations were found between tumor tissue Vav1 expression and peripheral blood Vav1 expression in both operation group and recurrent group (P < 0.05). Conclusions Vav1 expression increases in gastric cancer tissues and peripheral blood of patients with gastric cancer, and significant correlation is found between them. Vav1 level in the peripheral blood may serve as a new tumor marker to evaluate patients’ condition and predict recrudescence.