江西及周边地区神经系统疾病患者博尔纳病毒感染的分子流行病学研究

Molecular epidemiology of Borna virus infection in patients with nervous diseases in Jiangxi and its surrounding areas

摘要:

目的 探索博尔纳病毒（BDV）在江西及周边地区感染，导致神经系统疾病的分子流行病学状况。 方法 采用聚合酶链反应检测30 例病毒性脑炎（VE）的患者、10 例多发性硬化（MS）的患者、10 例急性 吉兰- 巴雷综合征（GBS）的患者、10 例帕金森病（PD）患者、60 例健康受试者外周血液中的单个核细胞 （PBMC）、60 只山羊的PBMC 中BDV p24 基因片段比较，对其阳性产物进行相关的基因序列测定，氨基酸排 列顺序及同源性的相关分析，并探讨其BDV 分子流行病学特点。结果 PBMC BDV p24 基因片段检出率比较， VE 和MS 患者高于健康受试者，差异有统计学意义（P <0.05），而山羊检测BDV p24 的基因片段阳性率为 1.67%。VE 和MS 患者的BDV p24 基因片段测序结果相一致，且与strain V 毒株同源性比较为95.73% ；而与 BDV/MDCK 毒株同源性比较为97.89%。山羊中测序BDV p24 基因片段结果与GenBank 提供的strain V 毒株 同源性比较为95.72%，与BDV/MDCK 毒株同源性比较为96.38% ；且与C6BV 毒株同源性比较为96.49%，但 其所编码的氨基酸没有实质性改变。结论 BDV 可能是新发感染性疾病中的一种病原体，与人类某些神经系 统疾病（如VE、MS）的发生可能存在密切关系，江西及周边地区某些神经系统疾病患者中BDV 感染可能存 在动物源性，尚待进一步深入研究及分析，从而提高对该类疾病的认识，以便更有效地对其进行防治。

Abstract:

Objective To investigate the epidemic situation of Borna virus (BDV) infection in Jiangxi and the surrounding areas. Methods Nested reverse transcriptase polymerase chain reaction (nRT-PCR) combined with fluorescence quantitative PCR (qRT-PCR) was used to detect BDV p24 gene fragment of the peripheral blood mononuclear cells (PBMCs) in 30 patients of viral encephalitis (VE), 10 patients of multiple sclerosis (MS), 10 patients of acute Guillain Barre syndrome (GBS), 10 patients with Parkinson’s disease (PD), 60 healthy people, and 60 goats. Keegan sequence and homology analysis of the positive products were carried out, and the characteristics of BDV molecular epidemiology were analyzed. Results The positive rate of BDV p24 gene fragment in the VE patients and the MS patients were significantly higher than that in the healthy subjects (P < 0.05), while the BDV p24 gene fragment positive rate in the goats was 1.67%. The sequence of BDV p24 gene fragment was consistent in the VE patients and the MS patients, and the homology with strain V was 95.73%, and the homology with BDV/MDCK strain was 97.89%. When the results of BDV p24 gene sequencing in the goats were compared with that of strain V provided by GenBank, the homology was 95.72%; when compared with that of BDV/MDCK strain, the homology was 96.38%; when compared with C6BV strain, the homology was 96.49%, but there was no substantial change in the amino acid encoded by the strain. Conclusions BDV may be a new pathogen of infectious diseases, and may be closely correlated with the occurrence of some neurological diseases (such as VE, MS) in human. There may be animal origin of BDV infection in some patients with nervous system diseases in Jiangxi and its surrounding areas. It needs further in-depth research and analysis, so as to improve the understanding of this kind of disease, and to prevent and control it more effectively.