高海拔地区藏族乳腺浸润性导管癌凋亡相关蛋白的表达及凋亡指数的研究

Expressions of apoptosis-related proteins and apoptosis index of Tibetan breast invasive ductal cancer in high altitude

摘要:

目的 探讨藏族乳腺浸润性导管癌Bcl-2、Bax 及Caspase 3 和凋亡指数（AI）的表达及临床意 义。方法 采用免疫组织化学法研究60 例世居藏族乳腺浸润性导管癌与60 例藏族乳腺纤维瘤石蜡组织切片 中Bcl-2、Bax 和Caspase 3 蛋白的表达；采用原位DNA 缺口末端标记法检测凋亡指数。结果 藏族乳腺浸 润性导管癌Bcl-2、Bax 及Caspase 3 的表达率分别为51.7%、76.7% 及60.0%，藏族乳腺纤维瘤组织的Bcl-2、 Bax 及Caspase 3 表达率分别为81.7%、85.0% 和80.0%，乳腺浸润性导管癌与乳腺纤维瘤Bcl-2 和Caspase 3 表 达率比较，差异有统计学意义（P <0.05），乳腺纤维瘤组织高于乳腺浸润性导管癌。乳腺浸润性导管癌中AI 阳性率与乳腺纤维瘤比较，差异有统计学意义（P <0.05），乳腺浸润性导管癌低于乳腺纤维瘤。AI 与肿瘤直径、 TNM 分期、孕激素受体（PR）表达相关（P <0.05），乳腺浸润性导管癌Bcl-2 的表达与淋巴结转移、雌激 素受体（ER）表达相关（P <0.05），Caspase 3 的表达与ER 表达相关（P <0.05）；乳腺浸润性导管癌中Bcl-2 蛋白表达与Bax、Caspase 3 蛋白表达相关（P <0.05）。结论 藏族乳腺浸润性导管癌中Bcl-2、Caspase 3 蛋白 低表达；Bcl-2 的表达与Bax、Caspase 3 的表达相关；Bcl-2 的表达与淋巴结转移、ER 表达相关；藏族乳腺 浸润性导管癌中AI 低表达与凋亡蛋白Bcl-2、Bax、Caspase 3 的表达无关； AI 与肿瘤直径、TNM 分期、PR 表达相关。

Abstract:

Objective To investigate the expressions of Bcl-2, Bax, caspase 3 and apoptotic index (AI) in Tibetan breast invasive ductal cancer, and analyze their correlations with clinicopathological characteristics. Methods The expressions of Bcl-2, Bax and caspase 3 were examined with immunohistochemical stain in histopathologic slides of 60 cases of Tibetan breast invasive ductal cancers and 60 cases of Tibetan breast fibrocellular tumors, and AI was detected by TUNEL method. Results The positive expression rates of Bcl-2, Bax, caspase 3 were 52.2%, 76.7% and 60.0% in Tibetan breast invasive ductal cancer and 81.7%, 85.0% and 80.0% in Tibetan fibrocellular tumor separately. The positive expression rates of Bcl-2 and caspase 3 in Tibetan breast invasive ductal cancer were lower than those in Tibetan breast fibroma tissues (P < 0.05). The AI of Tibetan breast invasive ductal cancer was lower than that of Tibetan breast fibrocellular tumor tissues (P < 0.05). The AI was related to the diameter of tumor, TNM stage and PR in Tibetan breast invasive ductal cancer (P < 0.05). Bcl-2 expression in Tibetan breast invasive ductal cancer was related to the lymph node involvement and ER (P < 0.05), caspase 3 was related to the ER (P < 0.05). Bcl-2 was correlated with Bax and caspase 3 in Tibetan breast invasive ductal cancer (P < 0.05). Conclusions The expressions of Bcl-2 and caspase 3 in Tibetan breast invasive ductal cancer are lower than those in Tibetan breast fibrocellular tumor. Bcl-2 expression is correlated with Bax and caspase 3 in Tibetan breast invasive ductal cancer. The expression of Bcl-2 is also related with the lymph node involvement and ER. Apoptotic index is lower in Tibetan breast invasive ductal cancer than in breast fibrocellular tumor. AI has correlations with tumor size, TNM stage and PR, but has no correlation with the expression of Bcl-2, Bax or caspase 3 in Tibetan breast invasive ductal cancer.