甲型肝炎患者黏蛋白域蛋白1 和4 表达与抗体产生的相关性研究

Effect of TIM-1 and TIM-4 on immunity in recovery stage of hepatitis patients

摘要:

目的 探讨甲型肝炎患者恢复期T 细胞免疫球蛋白及黏蛋白域蛋白（TIM）1 和4 mRNA 水平与 体液免疫的关系，了解TIM-1 和TIM-4 在体液免疫抗体产生过程中的相关作用。方法 选取甲型病毒性 肝炎典型病程的60 例患者于恢复期留取血液标本，以同期60 例健康体检志愿者血液标本作为对照，qRTPCR 检测TIM-1 和TIM-4 mRNA 水平，ELISA 检测外周血白介素4（IL-4）、白介素10（IL-10）、肿瘤坏 死因子α（TNF-α）和血清总Ig（IgG、IgM 和IgA）水平。结果 甲型病毒性肝炎患者外周血TIM-1 及 TIM-4 mRNA 的表达水平高于对照组（P <0.05）；甲型病毒性肝炎患者血清中IL-4 和IL-10 水平高于对 照组（P <0.05）；甲型病毒性肝炎患者血清总Ig（IgG、IgM 和IgA）水平高于正常对照组（P <0.05）。结论 TIM-1 及TIM-4 水平升高可能通过提高IL-4、IL-10 和TNF-α 来增强体液免疫的功能。

Abstract:

Objective To study the correlation between T cell immunoglobulin domain and mucin domain-1 (TIM-1) and TIM-4 and humoral immunity in recovery stage of hepatitis patients. Methods A total of 60 hepatitis patients on recovery period were chosen, and 60 healthy volunteers were involved in control group. qRT-PCR were utilized to measure expression levels of TIM-1 and TIM-4. Levels of IL-4, IL-10, TNF-α and total serum Ig were identified by ELISA. Results Expression levels of TIM-1 and TIM-4 were upregulated significantly in hepatitis patients compared with control group (P < 0.05). Serum IL–4, IL–10 and total Ig increased significantly in hepatitis patients when compared with control group (P < 0.05). Conclusion TIM-1 and TIM-4 may enhance the humoral immunity by upregulating cytokines such as IL-4 and IL-10.