初发急性白血病患者骨髓乳腺癌耐药蛋白基因表达及其与预后关系的研究

Prognostic significance of breast cancer resistance protein in primary acute leukemia patients

摘要:

目的 研究初发急性白血病患者骨髓乳腺癌耐药蛋白（BCRP ）基因表达及其与预后关系的相关性。 方法 选取2014 年1 月-2014 年12 月入住该院血液内科的急性白血病患者67 例，同时选择50 例健康志愿者 作为对照组。通过实时荧光定量PCR 检测骨髓BCRP 基因表达，并分析比较骨髓BCRP 基因表达和临床预后的 关系。结果 急性白血病患者骨髓BCRP 基因表达高于对照组（P <0.01），按照BCRP 均值（3.0）将患者分 为BCRP 高表达组和BCRP 低表达组。BCRP 高表达组患者初始WBC 计数较BCRP 低表达组高（P <0.01）， 且两组生存分析比较差异有统计学意义（Log-rank=21.78，P <0.05）。结论 急性白血病患者骨髓BCRP 基因表 达高于健康志愿者，且BCRP 高表达患者的预后更差。

Abstract:

Objective To investigate the gene expression of breast cancer resistance protein (BCRP) in bone marrow of acute leukemia (AL) and its prognosis. Methods A total of 67 AL patients admitted in our hospital from January 2014 to December 2014 were enrolled in the study, and 10 healthy volunteers were involved as control group. Real-time PCR was utilized to measure the gene expression of BCRP in bone marrow. And the difference in the BCRP gene expression and its prognosis were analyzed. Results The gene expression of BCRP in bone marrow of AL patients was statistically higher than that in the healthy volunteer group (P < 0.01). Acute leukemia patients were divided into high BCRP group and low BCRP group according to the mean of BCRP (3.0). Compared with low BCRP group, the primary WBC count was higher in BCRP high group (P < 0.01). The survival analysis between the two groups was statistically significant (Log-rank = 21.78, P < 0.05). Conclusion The gene expression of BCRP in bone marrow of AL patients was statistically increased and associated with the prognosis. High BCRP gene expression was a risk factor for poor prognosis in patients with AL.