妊娠糖尿病患者血清中RBP4 及NF-κB活性的变化及临床意义

Changes of serum RBP4 and NF-κB in patients with gestational diabetes mellitus and its clinical significance

摘要:

目的 探究视黄醇结合蛋白4（RBP4）和蛋白质核因子κB（NF-κB）在妊娠糖尿病患者外周血 中的表达及其临床意义。方法 选取2014 年12 月-2016 年12 月在该院产科产检的104 例孕妇，依据其糖耐 量检测结果分为妊娠糖尿病（GDM）组46 例和妊娠正常糖耐量（NGT）组58 例，通过ELISA 测定两组血清中 RBP4、NF-κB、空腹血糖（FBG）及空腹血胰岛素（Fins）等指标的变化，并分析其与胰岛素抵抗（HOMAIR） 的相关性。结果 妊娠糖尿病组血清中RBP4、NF-κB、FBG 及HOMA-IR 等指标均高于妊娠正常糖耐 量组（P <0.05）。血清中NF-κB 的活性与HOMA-IR 呈正相关（r =0.755，P =0.003）；血清中RBP4 的水 平与HOMA-IR 不相关（r =0.268，P =0.376）；RBP4 与NF-κB 的水平呈正相关（r =0.752，P =0.003）。结 论 RBP4 水平和NF-κB 活性在妊娠糖尿病的发生、发展中起重要作用，可能为其治疗提供潜在的靶点。

Abstract:

Objective To explore the clinical significance of the RBP4 and NF-κB in serum of patients with gestational diabetes mellitus. Methods Totally 104 pregnant women were collected in our hospital from December 2014 to December 2016, which were divided into gestational diabetes mellitus group (GDM) (46 cases) and normal glucose tolerance pregnancy group (NGT) (58 cases) according to the sugar tolerance test results. Changes of serum RBP4 level, NF-κB activity, fasting blood glucose (FBG) and fasting blood insulin (Fins) were detected by Enzyme-linked immunosorbent assay (ELISA). Correlations between insulin resistance (HOMA-IR) and them were analyzed. Result Compared with the GNT group, serum RBP4 level, NF-κB activity, FBG level and Fins level were significantly increased in GDM group (P < 0.05). The serum NF-κB activity was positively correlated with HOMA-IR (r = 0.755, P = 0.003); there was no significant correlation between the serum BRP4 level and HOMA-IR (r = 0.268, P = 0.376); the serum BRP4 level had a positive correlation with NF-κB activity (r = 0.752, P = 0.003). Conclusion BRP4 level and NF-κB activity may play an important role in gestational diabetes mellitus process, and they are likely to be potential targets for treatment of gestational diabetes mellitus.