阿伦磷酸钠缓释微球局部抗骨质疏松效果研究

Local application of Alendronate sodium sustained-release microspheres in osteoporosis

摘要:

目的 探讨阿伦磷酸钠缓释微球对骨质疏松大鼠局部的缓释效果。方法 将50 只骨质疏松大鼠随 机分为4 组，A 组为对照组，不予任何干预；B 组为全身给药组，通过灌胃方式给药；C 组为空白微球组，D 组 为载药微球组，C、D 组均通过注射方式予左股骨中部注入微球。8 周后处死各组大鼠，取左股骨，D 组同时取 右侧股骨，测量各组大鼠左股骨骨密度，并测量D 组大鼠右股骨骨密度。同时对各组大鼠左侧股骨骨应力进行 测量比较，并对D 组大鼠右侧股骨进行骨应力测量。结果 B 组、D 组较A 组骨密度增加（P <0.05），C 组与A 组骨密度差异无统计学意义（P >0.05），D 组骨密度高于B 组（P <0.05）。D 组左侧股骨骨密度高于右侧（P <0.05）。 股骨应力学检测结果提示，B 组、D 组较A 组三点弯曲最大载荷值增加（P <0.05），C 组与A 组三点弯曲最大 载荷值差异无统计学意义（P >0.05），D 组三点弯曲最大载荷值高于B 组（P <0.05）。D 组左侧股骨三点弯曲 最大载荷值高于右侧（P <0.05）。结论 应用阿伦磷酸钠缓释微球可增强骨质疏松大鼠局部的骨密度。

Abstract:

Objective To investigate local application of Alendronate sodium sustained release microspheres (ASSRM) on osteoporosis and its therapeutic effect. Methods A total of 40 osteoporotic rats were randomly divided into 4 groups: group A in which rats did not receive any intervention, group B in which drugs were systemically administrated, group C in which rats received injection of blank microsphere at left femur, and group D in which drug loaded microsphere group were injected into the left of femur. Femurs were harvested 8 weeks after treatments. Bone density was measured. Bone stress was manifested by maximum load of three-point bending. Results No obvious difference in bone density and stress was observed between group C and A (P > 0.05). Rats in group B and group D experienced increased bone density and stress compared with group A (P < 0.05), while femurs in group D had improved bone density and stress when compared with that in group B (P < 0.05). Inside group D, ASSRM treated femurs exerted significant upregulation of bone density and stress when compared with contralateral right femur (P < 0.05). Conclusion Application of Alendronate sodium sustained release microspheres significantly enhances the local bone mineral density and bone stress in osteoporosis.