FOXO3a 与乳腺浸润性导管癌血管生成的相关性

Effect of FOXO3a on angiogenesis in breast invasive ductal carcinoma

摘要:

目的 探讨转录因子FOXO3a 的表达与乳腺浸润性导管癌血管生成的相关性。方法 选择该院行手术 切除的乳腺浸润性导管癌组织（乳腺癌组）及相应的癌旁正常组织（癌旁组）各102 例，用免疫组织化学法（免 疫组化）检测FOXO3a 在两组中的表达情况，用CD34 标记微血管的内皮细胞，测定微血管密度（MVD），并 分析FOXO3a 在乳腺癌组织细胞质、细胞核的不同定位与MVD 的关系。结果 ① FOXO3a 在乳腺癌中的表达 （70.59%，72/102）低于癌旁组织（84.31%，86/102）（P <0.05）。FOXO3a 在乳腺癌组织细胞核表达的阳性率为 （15.69%，16/102），低于癌旁组织（81.37%，83/103）（P <0.05）；而在细胞质表达的阳性率（63.73％，65/102） 高于癌旁组（14.71%，15/102）（P <0.05）；②乳腺癌组织中MVD 的水平（27.46±5.87）高于癌旁组（17.25±3.61） （P <0.05）。乳腺癌组织细胞核FOXO3a 阳性者MVD 的水平（23.19±4.00）低于FOXO3a 阴性者（28.24±5.84） （P <0.05）；细胞质FOXO3a 阳性者MVD 的水平（26.39±3.12）与FOXO3a 阴性者（27.90±4.37）比较，差 异无统计学意义（P >0.05）。结论 FOXO3a 在细胞核的表达增加可能对乳腺癌组织中微血管的生成起抑制作用。

Abstract:

Objectives To investigate the correlation between expression of FOXO3a and angiogenesis in breast invasive ductal carcinoma. Methods A total of 102 cases of breast invasive ductal carcinoma tissue (cancer group) and nearby normal tissue group (peri-carcinoma group) were involved in this study. Expression of FOXO3a was detected by immunohistochemistry. CD34 was stained as a marker of microvascular endothelial cells. Microvessel density (MVD) was evaluated and correlation between MVD and FOXO3a was analyzed. Results Expression of FOXO3a in breast cancer cells was significantly lower than that of peri-carcinoma group (70.59% vs 84.31%, P < 0.05). The positive rate of FOXO3a in nucleus of breast cancercells was decreased (15.69% vs 81.37%, P < 0.05) while that in cytoplasm increased dramatically (63.73% vs 14.71%, P < 0.05) compared with peri-carcinoma group. MVD in breast cancer tissues and cancer tissues with expression of FOXO3a in nucleus was upregulated obviously when compared with peri-carcinoma group and cancer tissues without expression of FOXO3a in nucleus, respectively [(27.46 ± 5.87 vs 17.25 ± 3.61, P < 0.05), (23.19 ± 4.00 vs 28.24 ± 5.84, P < 0.05), respectively]. MVD in cancer tissues with expression of FOXO3a in cytoplasm was not significantly different compared with that in cancer tissue cells without expression of FOXO3a in cytoplasm (26.39 ± 3.12 vs 27.90 ± 4.37, P > 0.05). Conclusion Expression of FOXO3a in nucleus of cancer tissue cells plays an important role in angiogenesis inhibition in breast invasive ductal carcinoma.