MicroRNA-153 对脑膜瘤细胞放疗敏感性的影响

Effect of miR-153 on radiosensitivity of meningioma cells

摘要:

目的 探讨microRNA-153 对（miR-153）对脑膜瘤细胞放疗敏感性的影响及其作用机制。 方法 采用实时荧光定量聚合酶链反应检测放疗前后脑膜瘤SF3061 细胞系中miR-153 的表达变化，用脂质 体转染技术将miR-153 mimics 及对照转染至SF3061 细胞中，采用MTT 法、流式细胞术及克隆形成实验检 测放射处理后细胞的放疗敏感性变化。使用TargetScan 预测及双荧光素酶报告基因实验验证miR-153 与视 网膜母细胞瘤蛋白结合锌指1（RIZ1）的靶向作用，基因敲除RIZ1 验证其对脑膜瘤细胞放疗敏感性的影响。 结果 脑膜瘤细胞经过放疗处理后miR-153 的表达水平降低；miR-153 mimics 提高放疗后细胞的增殖抑制 率和凋亡率，降低细胞克隆形成率；TargetScan 预测及双荧光素酶报告基因实验证实RIZ1 是miR-153 的靶标； 转染si-RIZ1 增加脑膜瘤细胞的放疗敏感性。结论 miR-153 通过靶向干扰RIZ1 的表达，增加脑膜瘤细胞 的放疗敏感性。

Abstract:

Objective To investigate the effect of miR-153 on meningioma SF3061 cells and the molecular mechanism. Methods qRT-PCR was used to detect the expression levels of miR-153 in SF3061 cells treated or untreated with radiation. High expression of miR-153 was performed by transfecting miR-153 mimics into the SF3061 cells. MTT assay, flow cytometry and colony formation assay were used to determine cell growth, apoptosis and colony formation ability of meningioma cells treated with radiation, respectively. The relationship of miR-153 and RIZ1 was determined by the TargetScan and dual luciferase reporter. Si-RIZ1 was used to detect the effect of RIZ1 on radiosensitivity of meningioma cells. Results Radiation treatment significantly decreased the expression level of miR-153. miR-153 mimics obviously increased the cell growth inhibition rate and the apoptosis rate, and decreased the colony formation ability in the SF3061 cells treated with radiation. Results from the TargetScan and dual luciferase reporter showed that RIZ1 was the target of miR-153. Furthermore, knockdown of RIZ1 enhanced the radiosensitivity of the meningioma cells. Conclusions miR-153 enhances radiosensitivity of meningioma cells by targeting RIZ1.