CD 44 基因多态性对原发性肝癌细胞 局部T 细胞亚群浸润的影响

Effect of CD44 gene polymorphism on infiltration of local T cell subsets in primary liver cancer tissue

摘要:

目的 探讨CD 44 基因多态性对原发性肝癌细胞局部T 细胞亚群浸润的影响。方法 前瞻性收 集2017 年1 月-2017 年12 月吉林大学第一医院收治的原发性肝癌患者109 例，根据CD 44 基因rs4756195 位 点单核苷酸多态性，将患者分为AA 组、AG 组和GG 组，观察3 组患者局部组织T 细胞亚群浸润情况。采 用流式细胞术检测CD4+ T、CD8+ T、Treg 及Th17 细胞，免疫组织化学法检测白介素-2（IL-2）、IL-10 肿瘤生长因子-β（TGF-β）和IL-17 的阳性表达，聚合酶链反应检测CD 44 基因rs4756195 位点单核苷酸 多态性。结果 AA 组、AG 组、GG 组CD4+ T 细胞分别为（21.08±2.12）%、（19.43±1.98）%、（19.12±2.09）%， 差异有统计学意义（P <0.05）；CD8+ T 细胞分别为（28.83±2.52）%、（25.52±2.71）%、（24.91±2.29）%， 差异有统计学意义（P <0.05）；调节性T 细胞分别为（6.12±1.47）%、（8.12±1.71）%、（8.24±1.92）%， 差异有统计学意义（P <0.05）；IL-2 阳性表达率分别为82.09%、47.83%、68.42%，差异有统计学意义（P <0.05）； IL-10 阳性表达率分别为46.27%、69.57%、73.68%，差异有统计学意义（P <0.05）；TGF-β 阳性表达率分 别为41.79%、77.27%、78.95%，差异有统计学意义（P <0.05）。结论 原发性肝癌患者CD 44 基因rs4756195 位点呈多态性分布，与肝癌组织中的免疫抑制有关。

Abstract:

Objective To investigate the effect of CD44 gene polymorphism on the local infiltration of T cell subsets in primary liver cancer tissue. Methods From January to December 2017, 109 cases of primary liver cancer patients were prospectively collected in our hospital. According to the CD44 gene rs4756195 single nucleotide polymorphism (SNP), the patients were divided into group AA, group AG and group GG. The local tissue infiltration of T cell subsets of the three groups was studied. Flow cytometry was used to detect CD4+ and CD8+ T cells, Treg cells and Th17 cells. Immunohistochemical method was used to study the expressions of IL-2, IL-10, IL-17 and TGF-β. PCR was used to determine SNP of CD44 gene rs4756195. Results In the group AA, the group AG and the group GG, CD4+ T cells accounted for (21.08 ± 2.12)%, (19.43 ± 1.98)% and (19.12 ± 2.09)% respectively, there was significant difference (P < 0.05); CD8+ T cells accounted for (28.83 ± 2.52)%, (25.52 ± 2.71)% and (24.91 ± 2.29)% respectively, there was significant difference (P < 0.05); regulatory T cell occupied (6.12 ± 1.47)%, (8.12 ± 1.71)% and (8.24 ± 1.92)% respectively, the difference was statistically significant (P < 0.05); the positive expression rate of IL-2 was 82.09%, 47.83% and 68.42% respectively, the difference was statistically significant (P < 0.05); the positive expression rate of IL-10 was 46.27%, 69.57% and 73.68% respectively, the difference was statistically significant (P < 0.05); the positive expression rate of TGF-β was 41.79%, 77.27% and 78.95% respectively, the difference was statistically significant (P < 0.05). Conclusions The polymorphic distribution of CD44 gene rs4756195 locus in the patients with primary liver cancer is related to the immunosuppression in the liver cancer tissue.