MASP-2 在大肠癌患者血清及组织中的 表达及其意义

Expression of MASP-2 in serum and tissues of patients with colorectal cancer and its significance

摘要:

目的 研究人甘露糖结合凝集素相关丝氨酸蛋白酶2（MASP-2）在大肠癌患者外周血中的变 化及组织中的表达，为临床诊断和治疗提供参考。方法 采用酶联免疫吸附法检测MASP-2 含量并结合临床 资料进行分析，免疫组织化学法检测癌组织和癌旁组织的表达情况。结果 癌症组患者血清MASP-2 浓度低 于对照组（P <0.05），低分化人群MASP-2 浓度高于高- 中分化人群（P <0.05）。癌症组不同年龄、性别、 TNM 分期、淋巴结转移及癌症类型患者MASP-2 比较无差异（P >0.05）。癌症组患者MASP-2 阳性率高于 对照组患者（P <0.05）。结论 MASP-2 在早期大肠癌患者外周血中的浓度发生了变化，同时MASP-2 在肿 瘤组织中高表达，可能在其肿瘤的发生发展中起到一定作用。

Abstract:

Objective To investigate the content changes of human mannose-binding lectin associated serine protease 2 (MASP-2) in peripheral blood and tissues in patients with colorectal cancer and provide reference information for clinical diagnosis and treatment. Methods The content of MASP-2 was measured by enzyme-linked immunosorbent assay and its expression in colorectal carcinoma tissues and the adjacent tissues was detected by immunohistochemical method. Moreover, combined with the clinical data of the patients, all the results were analyzed at a later stage. Results The content of MASP-2 in serum of the patients with colorectal cancer was significantly lower than that of healthy people (P < 0.05). In addition, the concentration of MASP-2 in the group of patients with poorly-differentiated cancer was higher than that in the group of patients with moderate- or well-differentiated cancer. But there was no obvious difference in the concentration of MASP-2 between the cancer patients with different age, genders, TNM stages, lymphatic metastasis or cancer types (P > 0.05). The positive rate of MASP-2 in the patients with colorectal cancer was higher than that in the normal poeple (P < 0.05). Conclusions The serum expression of MASP-2 was obviously changed in the patients with early-stage colorectal cancer. In addition, it was higher in the cancer tissues than in the paracancerous normal tissue. Collectively, the results showed that MASP-2 maybe play an important role in the oncogenesis and progression of colorectal cancer.