梗阻性肾病肾组织中磷酸化 黏着斑激酶的表达及意义

Expression and significance of phosphorylated FAK in renal tissue of obstructive nephropathy

摘要:

目的 探讨梗阻性肾病肾组织中磷酸化黏着斑激酶（pFAK）的表达变化及意义。方法 行单侧输 尿管结扎术复制大鼠梗阻性肾病模型（UUO 模型）。分为假手术组，3 d、14 d 模型组共3 组，收集大鼠肾组织， 石蜡切片行免疫组织化学检测，新鲜标本行WB，检测各组大鼠肾组织pFAK、α- 平滑肌肌动蛋白（α-SMA） 和整合素β1（CD29）的表达。结果 假手术组肾组织pFAK 表达量低，3 d 模型组肾组织pFAK 表达升高，14 d 模型组较3 d 模型组升高。α-SMA、整合素β1 的表达与pFAK 一致。pFAK 的表达分别与α-SMA 及整合素 β1 的表达呈正相关。结论 pFAK 在大鼠梗阻性肾病肾间质纤维化中表达升高，FAK 可能在梗阻性肾病肾间 质纤维化过程中发挥促进作用，并可能是作为整合素β1 的下游，通过促进形成肌成纤维细胞而实现。

Abstract:

Objective To investigate the expression and significance of FAK on renal fibrosis of obstructive nephropathy. Methods Unilateral ureteral ligation was performed to establish rat model of obstructive nephropathy (UUO model). The rats were divided into 3 groups: sham group, injury group (3 days) and injury group (14 days). The expression of FAK, α-SMA and integrin β1 (CD29) in renal tissue of rats were detected by immunohistochemistry and western blot. Results Immunohistochemical staining and western blot showed that expression of FAK, α-SMA and integrin β1 in injury group (3 d) was significantly increased compared with that in sham group (P < 0.05) while expression of FAK in injury group (14 d) was higher than that in injury group (3 d) (P < 0.05). Conclusion Increased expression of p-FAK in renal tissue promotes fibrosis through integrin β1 mediated formation of myofibroblast.