肺鳞状细胞癌组织中血管扩张刺激磷蛋白的 临床意义及生物学功能研究

Clinical role and biofunctions of VASP in human lung squamous cell carcinoma

摘要:

目的 探讨肺鳞状细胞癌（LSCC）组织中血管扩张刺激磷蛋白（VASP）的临床意义及对肺癌细胞 迁移侵袭的影响。方法 检测50 例LSCC 及其癌旁组织中VASP 的表达，分析肿瘤组织VASP 表达与患者临 床特征及预后间的相关性。通过siRNA 沉默肺癌细胞中VASP 的表达，采用划痕愈合试验及Transwell 小室 模型检测沉默VASP 后肺癌细胞迁移及侵袭能力的变化。结果 肺癌组织中VASP 的阳性表达率（χ2=7.853， P =0.005）及阳性表达程度（t =4.318，P =0.000）均高于对应癌旁组织，并与肿瘤淋巴结转移（χ2=6.455，P = 0.011）及高TNM 分期呈正相关（χ2=6.455，P =0.011）；肺癌组织VASP 蛋白表达阳性患者3 年总生存率（χ2= 8.311，P =0.004）及无病生存率（χ2=9.969，P =0.002）均低于VASP 蛋白表达阴性患者。在体外，沉默 VASP 的表达能够抑制肺癌细胞的迁移（t =9.021，P =0.011）及侵袭（t =4.609，P =0.041）能力。结论 肺鳞 癌组织中VASP 表达升高并与肿瘤患者较差的生存获益有关，沉默VASP 表达在体外能够实现一定的抗肿瘤 转移效应。

Abstract:

Abstract: Objective To investigate the expression of vasodilator stimulated phosphoprotein (VASP) and its clinical significance in human lung squamous cell carcinoma (LSCC). Methods The expression of VASP in 50 LSCC tissues and matched tumor adjacent tissues was detected by immunohistochemistry. The relationship between VASP and clinical features were determined by chi-square test. VASP was knocked downby siRNA technologyin vitro. Results The expression of VASP was up-regulated in tumor tissues than non-tumor tissues (χ2 = 7.853, P = 0.005; t = 4.318, P =0.000). VASP was positively associated with lymphatic metastasis (χ2 = 6.455, P =0.011) and advanced TNM stage ( Ⅲ + Ⅳ stage, χ2 = 6.455, P = 0.011). Three-year overall survival rate and disease-free survival rate were dramatically lower in VASP positive group than those in VASP negative expression group (χ2 = 8.311, P = 0.004, χ2 = 9.969, P = 0.002). Knockdown of VASP inhibited the migration and invasion of cancer cells in vitrocondition. Conclusions Expression of VASP is significantly increased in LSCC tissues and may be involved in tumor metastasis.