小鼠肾发育中Slit2 及其受体Robo1 的表达

Expressions of Slit2 and its receptor Robo1 during development of mouse kidneys

摘要:

目的 观察并检测Slit2 及其受体Robo1 在小鼠肾发育中的表达变化。方法 通过免疫组织化学技 术系统观察小鼠肾脏Slit2 和Robo1 的表达和定位；应用蛋白印迹技术检测小鼠肾脏Slit2 和Robo1 表达的变 化规律。结果 Slit2 和Robo1 在胚龄12 d 小鼠肾脏生肾区开始表达；Slit2 在小鼠输尿管芽及其周围的间充质 聚集的部位阳性表达，Robo1 在小鼠肾脏生肾区广泛表达，主要分布于生后肾组织和输尿管芽上皮细胞基底 面；在肾单位发育过程中，Slit2 定位表达于逗号小体阶段和S 小体阶段，且表达较强，在Ⅲ期肾小体阶段、Ⅳ 期肾小体阶段及成熟肾小体阶段有微弱表达；Robo1 定位表达于逗号小体阶段、S 小体阶段、Ⅲ和Ⅳ期肾小 体阶段，且表达较强，在成熟肾小体阶段有微弱表达；Slit2 及其受体Robo1 在肾脏泌尿小管如近端小管、远 端小管及集合管发育过程中均有表达。蛋白印迹检测从小鼠胚龄14 d 开始，Slit2 表达先递增，至胚龄16 d 达 峰值，而后逐渐递减。Robo1 表达从小鼠胚龄14 d 开始逐渐递减。结论 Slit2 及其受体Robo1 参与肾输尿管 芽的萌出、输尿管芽与生后肾组织的诱导、肾小体发育及泌尿小管发育和成熟过程。

Abstract:

Objective To study the expression changes of Slit2 and its receptor Robo1 in the kidneys of mice at different developmental stages. Methods The expressions of Slit2 and Robo1 were examined by immunohistochemical staining and Western blot. Results Slit2 and Robo1 began to be expressed in the nephrogenic regions of the mouse kidneys at the embryonic age of 12 days. Among them, Slit2 was positively expressed in the ureteric bud and the surrounding mesenchyme but not in other parts of the mice, while Robo1 was widely expressed in nephrogenic zone of the mice, mainly in the metanephrogenic tissue and the basal surface of the ureteric bud epithelium. In the developmental process of nephrons, Slit2 was strongly expressed in the comma body and S body stages, and weakly expressed in the stage III of renal corpuscle, the stage IV of renal corpuscle and the mature stage of renal corpuscle in the mice. Robo1 was strongly expressed in the stages of comma body and S body, the stage III of renal corpuscle and the stage IV of renal corpuscle, but weakly expressed in the mature stage of renal corpuscle in the mice. Both Slit2 and Robo1 were detected in proximal tubules, distal tubules and collecting ducts of the mice. The expression of Slit2 in the mice increased from the 14 day of embryo, reached the peak on the 16th day of embryonic age, and then gradually decreased. The expression of Robo1 protein in the mice gradually decreased from the 14th day of embryonic age. Conclusions The expressions of Slit2 and its receptor Robo1 in the renal corpuscles of developing stages and in the development and maturation of the uriniferous tubules in mice suggests that they may play an important role in the development of mouse renal corpuscles and uriniferous tubules.