食管癌组织中VEGF-C 表达量与淋巴管新生及 肿瘤组织生长的相关性

Correlation of VEGF-C expression with lymph vessel regeneration and tumor growth in esophageal carcinoma

摘要:

目的 研究食管癌组织中血管内皮生长因子C（VEGF-C）表达量与淋巴管新生、肿瘤组织生长的 相关性。方法 选取2012 年4 月-2015 年10 月在河北大学附属医院手术切除的食管癌组织作为临床标本，采 用免疫组织化学试剂盒对食管癌组织中VEGF-C、D2-40 进行染色，判读VEGF-C 的阳性表达率以及D2- 40 阳性表达的淋巴管密度（LVD）；采用荧光定量聚合酶联反应试剂盒测定Ki-67、Cyclin B1 的mRNA 含量。 结果 低未分化、TNM Ⅲ、Ⅳ、有淋巴结转移的食管癌组织中VEGF-C 阳性率、LVD 以及Ki-67、Cyclin B1 的mRNA 含量均高于中高分化、TNM Ⅰ、Ⅱ期、无淋巴结转移的食管癌组织；VEGF-C 阳性表达的食管 癌组织中LVD 以及Ki-67、Cyclin B1 的mRNA 含量均高于VEGF-C 阴性表达的食管癌组织。结论 食管癌 组织中高表达的VEGF-C 与淋巴管新生以及肿瘤组织生长密切相关。

Abstract:

Objective To study the correlation of expression of vascular endothelial growth factor C (VEGF-C) with lymph vessel regeneration and tumor growth in esophageal carcinoma. Methods Esophageal cancer tissues from surgical resection in our hospital from April 2012 to October 2015 were collected as clinical specimens. VEGF-C and D2-40 in the esophageal cancer tissues were stained by immunohistochemical kit, VEGF-C positive expression rate and lymphatic vessel density (LVD) with positive expression of D2-40 were judged. qRT-PCR was applied to determine mRNA content of Ki-67 and cyclin B1. Results The VEGF-C positive rate, LVD and mRNA content of Ki-67 and cyclin B1 in the poorly-differentiated and undifferentiated, TNM stages III and IV, lymph node metastatic esophageal cancer tissues were significantly higher than those in the medium- and well-differentiated, TNM stage I and II, no lymph node metastatic esophageal cancer tissues (P < 0.05). LVD and mRNA content of Ki-67 and cyclin B1 in the esophageal cancer tissues with positive expression of VEGF-C were significantly higher than those in the esophageal carcinoma tissue with VEGF-C negative expression (P < 0.05). Conclusions High expression of VEGF-C in esophageal cancer tissues is closely related to lymphatic regeneration and growth of tumor tissues.