不同复合应激致卵巢早衰大鼠的SRB1、 CYP2E1 蛋白表达水平比较

Comparison of expressions of SRB1 and CYP2E1 in rat models with premature ovarian failure induced by different combined stresses

摘要:

目的 比较卵巢早衰（POF）及中医寒邪、热邪复合应激大鼠的SRB1、CYP2E1 蛋白表达水平， 探讨其相互关系，为研究POF 的发生、发展提供依据。方法 选用50 只性功能正常的雌性Wistar 大鼠，随 机分为POF 组（A 组）、热邪复合应激模型组（B 组）、寒邪复合应激模型组（C 组），以及2 个正常对照组（N1 组、N2 组）。采用免疫组织化学法检测各组大鼠卵巢组织中SRB1、CYP2E1 蛋白的表达水平。结果 通过 各组大鼠生物学表征的定性和定量指标，以及相关中医专家的断定确认模型复制成功。与N1 组相比，A 组和 B 组SRB1、CYP2E1 表达升高（P <0.05）。与N2 组相比，C 组SRB1、CYP2E1 表达升高（P <0.05）。A 组与B、 C 组的SRB1、CYP2E1 表达水平比较，差异有统计学意义（P <0.05），B 组与C 组的SRB1、CYP2E1 表达水 平比较，差异无统计学意义（P >0.05）。结论 西医POF、热邪和寒邪复合应激大鼠的SRB1、CYP2E1 表达 水平有不同程度的变化。寒邪、热邪导致的POF 可能与体内胆固醇代谢异常及活性氧等物质的产生有一定 联系。与寒邪复合应激相比，热邪复合应激大鼠更易发生POF。

Abstract:

Objective To study the connection between premature ovarian failure (POF) and cold and heat pathogens with multiple stresses, establish the POF rat models by cold and heat pathogens with multiple stresses, to compare the expressions of SRB1 and CYP2E1 proteins in all groups, to provide the evidence of the study of occurrence and development of POF. Methods Fifty normal female Wistar rats with normal sexual function were selected and randomly divided into a POF group (group A), a heat pathogen combined with stress group (group B), a cold pathogen combined with stress (group C) and two normal control groups (N1 and N2). Immunohistochemical method was used to detect the expressions of SRB1 and CYP2E1 proteins in the ovarian tissues of the rats. Results Through determination of the qualitative and quantitative indicators of biological characterization of the rats in each group, the relevant experts confirmed the success of the models. Compared with the group N1, the expressions of SRB1 and CYP2E1 in the group A and the group B were significantly increased (P < 0.05). Compared with the group N2, the expressions of SRB1 and CYP2E1 in the group C were significantly increased (P < 0.05).The expressions of SRB1 and CYP2E1 in the group A were significantly different from those in the groups B and C (P < 0.05), but there was no significant difference in the expression of SRB1 or CYP2E1 between the group B and the group C (P > 0.05). Conclusions The expression levels of SRB1 and CYP2E1 in the rats with POF induced by different combined stresses of cold and heat pathogens vary in different degree. It suggests that POF in the rats may be related to the abnormal metabolism of cholesterol and the production of reactive oxygen species. Compared with cold pathogen combined stress, heat pathogen combined stress is more likely to cause POF.