薯蓣丸对化疗4T1 乳腺癌小鼠 T 淋巴细胞亚群的影响

Effect of Dioscorea pills on T cell subgroups in mice with 4T1 breast cancer after chemotherapy

摘要:

目的 评价薯蓣丸对化疗后4T1 乳腺癌小鼠免疫功能的影响，并探讨其作用机制。方法 随机 将32 只BALB/c 小鼠分为对照组、模型组、化疗组、中药组，每组8 只。除对照组外，其余组注射4T1 细胞 悬液复制荷瘤模型。成瘤后第1 天化疗组、中药组小鼠予以多西他赛治疗，1 次/7 d，共21 d ；注射多西他赛 后第2 天对中药组小鼠行中药灌胃，1 次/d，共21 d。治疗完成后处死小鼠取脾脏研磨，用流式细胞仪检测小 鼠脾脏中CD4 ＋、CD8 ＋、CD4 ＋ CD25 ＋ Foxp3 ＋调节性T 细胞（Treg）表达水平。结果 与模型组比较，化疗 组、中药组CD4 ＋、CD8 ＋、CD4 ＋ CD25 ＋ Foxp3 ＋ Treg 细胞表达水平降低，CD4 ＋ /CD8 ＋比值升高（P <0.05）； 与化疗组比较，中药组CD4 ＋细胞表达水平、CD4 ＋ /CD8 ＋比值升高，CD4 ＋ CD25 ＋ Foxp3 ＋ Treg 细胞表达水 平降低（P <0.05）。结论 薯蓣丸能改善化疗后乳腺癌小鼠免疫功能。

Abstract:

Objective To evaluate the effect of Dioscorea pills on the immunity of mice with 4T1 breast cancer after chemotherapy and its mechanism of action. Methods Thirty-two BALB/c mice were randomly divided into a control group, a model group, a chemotherapy group and a chemotherapy plus Chinese medicine group with 8 mice in each group. Except the control group, all the other groups were injected with a 4T1 breast cancer cell suspension to build a tumor-bearing mouse model. The chemotherapy group and the chemotherapy plus Chinese medicine group were treated with Docetaxel from the 1st day after tumor formation, once in 7 days, for 21 days; Dioscorea pills were given to the chemotherapy plus Chinese medicine group by gavage from the 2nd day after Docetaxel injection, once a day, for 21 days. After treatment, the mice were sacrificed and their spleens were taken out and ground, and then flow cytometry was used to detect the levels of CD4+, CD8+, CD4+CD25+Foxp3+ Treg cells. Results Compared with the model group, the levels of CD4+, CD8+ and CD4+CD25+Foxp3+ Treg cells decreased, while CD4+/CD8+ ratio increased in the chemotherapy group and the chemotherapy plus Chinese medicine group, and the differences were significant (P < 0.05). Compared with the chemotherapy group, the levels of CD4+ cells and CD4+/CD8+ ratio increased, while the level of CD4+CD25+Foxp3+ Treg cells decreased in the chemotherapy plus Chinese medicine group, and the differences were significant (P < 0.05). Conclusions Dioscorea pills can improve the immune function of mice with breast cancer after chemotherapy.