CDH17 对那可丁耐药性结肠癌细胞 裸鼠移植瘤模型的影响

Effect of CDH17 on transplanted tumor model of Noscapine-resistant colon cancer cells in nude mice

摘要:

目的 研究下调肝肠钙黏连蛋白（CDH 17）基因对那可丁耐药的结肠癌细胞裸鼠移植瘤模型的 影响。方法 以结肠癌细胞为研究材料，复制结肠癌细胞裸鼠移植瘤模型。同时构建干扰质粒，从基因和 蛋白水平对CDH17 进行干扰，成功构建慢病毒载体。通过转移酶介导的末端标记法（TUNEL）检测下调 CDH 17 基因对结肠癌细胞的影响。结果 实时聚合酶链反应和Western blot 检测证实成功构建CDH17 的干 扰表达载体；TUNEL 检测表明下调CDH 17 基因可以促进对那可丁耐药的结肠癌细胞裸鼠移植瘤模型细胞凋 亡。结论 下调CDH 17 基因可以促进那可丁耐药性结肠癌细胞凋亡，抑制肿瘤细胞增殖。

Abstract:

Objective To investigate the effect of down-regulation of CDH17 gene on the growth of transplanted tumor model of Noscapine-resistant colon cancer cells in nude mice. Methods The transplanted tumor model of colon cancer cells was established in nude mice. At the same time, the interference plasmid was constructed to control the expressions of CDH17 gene and protein. The effect of CDH17 gene down-regulation on colon cancer cells was detected by TUNEL method. Results RT-PCR and Western blot confirmed that the CDH17 interference expression vector was successfully constructed. TUNEL method showed that down-regulation of CDH17 gene promoted the apoptosis of Noscapine-resistant colon cancer cells of transplantation tumor model in nude mice. Conclusions Down-regulation of CDH17 gene can promote the apoptosis of Noscapine-resistant colon cancer cells and inhibit the proliferation of tumor cells.