雌激素水平对大鼠实验性牙周炎模型的影响

Effect of estrogen level on experimental periodontitis model in rats

摘要:

目的 研究雌激素水平对雌性大鼠实验性牙周炎模型牙周组织的影响。方法 取雌性未孕SD 大 鼠33 只，并随机分为假手术组（Sham 组）、去势组（OVX 组）和去势加雌激素组（OVX+E 组），适应性喂 养后对各组雌性大鼠行双侧卵巢切除术或假手术。OVX+E 组术后每3 天注射1 次雌二醇（E2）。利用钢丝 结扎右侧上颌磨牙的方法复制慢性牙周炎动物模型，左侧大鼠牙齿留作对照，不做处理。通过检测血清E2 水 平，观察临床表现和HE 切片，比较牙周结扎术后第2、4、6 周各组大鼠牙周炎的进展情况。结果 随着时间 的延长，OVX 组的E2 水平逐渐降低，Sham 组和OVX+E 组的E2 水平基本维持不变。各组牙周探诊程度逐 渐加深，OVX 组较Sham 组和OVX+E 组牙周炎较重。结论 雌激素水平的高低会影响牙周炎的病理发展过程， 内源性雌激素的缺乏会加重大鼠牙周炎的程度。

Abstract:

Objective To study the effect of estrogen on periodontal tissue of rats with experimental periodontitis. Methods Thirty-three female non-pregnant SD rats were randomly divided into a sham operation group (sham group), an ovariectomized group (OVX group) and an OVX+estrogen group (OVX+E group). After adaptive feeding, the female rats of each group underwent sham operation or bilateral oophorectomy respectively. Estradiol was injected postoperatively every three days in the OVX+E group. The animal model of chronic periodontitis was established by ligating the right maxillary molars with the wire, and the teeth of the left side of the rats were taken as control and not treated. Through the detection of serum E2 level, and observation of the clinical manifestations and HE sections, the development of periodontitis in the rats was compared after 2, 4 and 6 weeks. Results With the progress of time, the level of E2 in the OVX group decreased gradually, the level of E2 in the sham group and the OVX+E group remained unchanged. In each group, the level of periodontal probing gradually deepened, and the degree of periodontitis in the OVX group was higher than that in the sham group and the OVX+E group. Conclusions The level of estrogen can affect the pathological development of periodontitis, and the deficiency of endogenous estrogen may increase the degree of periodontitis.