冠心病患者外周血中EMMPRIN 表达量与斑块 特征、基质金属蛋白酶含量的关系研究

Relationships of peripheral blood EMMPRIN expression with plaque characteristics and MMPs content in patients with coronary heart disease

摘要:

目的 研究冠状动脉粥样硬化性心脏病（冠心病）患者外周血中细胞外基质金属蛋白酶诱导因子 （EMMPRIN）表达量与斑块特征、基质金属蛋白酶（MMP）含量的关系。方法 选取2013 年6 月-2015 年 10 月诊断为稳定型心绞痛（SAP）的69 例患者和急性冠状动脉综合征（ACS）的47 例患者分别纳入SAP 组 和ACS 组，选择同期体检的57 例健康志愿者纳入对照组，采用冠状动脉成像检查判断冠状动脉粥样斑块的 性质，采集外周血并测定EMMPRIN 的表达量以及MMP-1、MMP-2、MMP-3、MMP-9、MMP-14 的含 量。结果 SAP 组和ACS 组外周血单个核细胞表面EMMPRIN 的荧光强度以及血清中MMP-1、MMP-2、 MMP-3、MMP-9、MMP-14 的含量高于对照组，SAP 组外周血单个核细胞表面EMMPRIN 的荧光强度以 及血清中MMP-1、MMP-2、MMP-3、MMP-9、MMP-14 的含量高于ACS 组；SAP 组和ACS 组患者斑 块性质越不稳定，外周血单个核细胞表面EMMPRIN 的荧光强度以及血清中MMP-1、MMP-2、MMP-3、 MMP-9、MMP-14 的含量越高；外周血单个核细胞表面EMMPRIN 的荧光强度与血清中MMP-1、MMP- 2、MMP-3、MMP-9、MMP-14 的含量呈正相关。结论 冠心病患者外周血中EMMPRIN 表达量上调与粥 样斑块性质不稳定、MMPs 含量增加有关。

Abstract:

Objective To study the relationships of extracellular matrix metalloproteinase inducer (EMMPRIN) expression in peripheral blood of the patients with coronary heart disease with plaque characteristics and content of matrix metalloproteinases (MMPs). Methods In this study, 69 patients diagnosed as stable angina pectoris (SAP) and 47 patients diagnosed as acute coronary syndrome (ACS) from June 2013 to October 2015 were separately enrolled into the SAP group and the ACS group, 57 cases of healthy volunteers during the same period were enrolled in the control group. Characteristics of coronary atherosclerotic plaque were judged by coronary artery imaging; peripheral blood was collected, EMMPRIN expression and the content of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-14 were determined. Results EMMPRIN fluorescence intensity of peripheral blood mononuclear cells and serum levels of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-14 of the SAP group and the ACS group were significantly higher than those of the control group, EMMPRIN fluorescence intensity of peripheral blood mononuclear cells and serum levels of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-14 of the ACS group were significantly higher than those of the SAP group. The less stable the plaque properties, the higher the EMMPRIN fluorescence intensity of peripheral blood mononuclear cells and serum levels of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-14 of the SAP group and the ACS group. EMMPRIN fluorescence intensity of peripheral blood mononuclear cells was positively correlated with serum levels of MMP-1, MMP-2, MMP-3, MMP-9 and MMP-14. Conclusions Up-regulation of EMMPRIN expression in peripheral blood of the patients with coronary heart disease is related to the unstable atherosclerotic plaque and the increase of MMPs content.