大花红景天抗辐射活性成分的初步筛选

Screening of radiation-protective constituents of Rhodiola crenulata

摘要:

目的 评价大花红景天不同提取成分对受照射BALB/c 小鼠体重和外周血象的影响。方法 选 取6 ～ 8 周的BALB/C 小鼠60 只，随机分为正常组、对照组、乙醇提取物组、总黄酮组、乙醇提取其余成分组。 采用3.5 Gy 60Coγ 射线全身单次照射，复制小鼠电离辐射损伤模型，照射后连续7 d 灌胃给药。照射后1、4、7、 10、13 和16 d，观察大花红景天不同提取成分对小鼠体重、外周血象的影响。结果 与对照组比较，乙醇提取物、 总黄酮、乙醇提取其余成分可有效提高受照射小鼠的外周血白细胞数、红细胞数、血红蛋白含量和血小板数 （P <0.05）。乙醇提取物和乙醇提取其余成分提高受照射小鼠外周血红细胞数、血红蛋白含量的作用低于总黄 酮组（P <0.05）。大花红景天不同提取成分对受照射小鼠的体重无明显影响（P >0.05）。结论 总黄酮可能 是大花红景天主要抗辐射活性成分。

Abstract:

Objective To evaluate the radiation-protective effect of Rhodiola crenulata on BALB/c mice. Methods Sixty BALB/c mice aged 6-8 weeks were divided by random number table method into a normal control group, an irradiation control group, a Rhodiola crenulata ethanol extract group, a total flavonoids group, and other constituents group, respectively. Radiation-damaged mouse model was established by single irradiation of 3.5 Gy 60Co γ-rays. After irradiation different extracts of Rhodiola crenulata were administered intragastrically for 7 consecutive days. The activity indexes including body weight and peripheral hemogram were detected on the 1st, 4th, 7th, 10th, 13th and 16th day after irradiation. Results Compared to the irradiation control group, the peripheral blood WBC count, RBC count, HGB content and PLT count of the irradiated mice were increased greatly by the Rhodiola crenulata ethanol extract, the Rhodiola crenulata’s total flavonoids and the Rhodiola crenulata’s other constituents (P < 0.05). The effect of increasing peripheral blood RBC count and HGB content in the Rhodiola crenulata ethanol extract group and other constituents group was obviously weaker than that in the total flavonoids group (P < 0.05). Besides, different extracts of Rhodiola crenulata had no obvious effect on the body weight of irradiated mice (P > 0.05). Conclusions Probably, the total flavonoids are the radiation-protective constituents of Rhodiola crenulata .