HIF-2α 和Twist2 在乳腺癌中的 表达及其与预后的关系

Expressions of HIF-2α and Twist2 in breast carcinoma and their relationships with clinical prognosis

摘要:

目的 探讨缺氧诱导因子-2α（HIF-2α）和Twist2 在乳腺癌中的表达及其与预后的关系。 方法 采用免疫组织化学SP 法分别检测乳腺癌、纤维腺瘤及癌旁正常组织中HIF-2α、Twist2 的表达，并 对92 例乳腺癌患者的生存结局进行随访。结果 乳腺癌组HIF-2α、Twist2 的表达高于癌旁正常组织组及 纤维腺瘤组（P <0.05）。不同肿瘤大小、有无淋巴结转移乳腺癌患者的HIF-2α 阳性率比较有差异（P <0.05）， 不同组织学分级、有无淋巴结转移及雌激素受体乳腺癌患者的Twist2 阳性率比较有差异（P <0.05），乳腺 癌中HIF-2α 与Twist2 的表达呈正相关（P <0.05）。HIF-2α 和Twist2 阳性乳腺癌患者术后5 年生存率均 低于阴性患者（P <0.05）。结论 HIF-2α 和Twist2 在乳腺癌中高表达，两者共同促进肿瘤的浸润、转移， HIF-2α 高表达提示患者预后不良，可作为预测乳腺癌患者预后的影响因素之一。

Abstract:

Objective To investigate the expressions of HIF-2α and Twist2 in breast carcinoma and their relationships with tumor metastasis and prognosis. Methods The expressions of HIF-2α and Twist2 proteins in breast carcinoma, adjacent tissues and fibroadenoma were detected by immunohistochemical SP method. Survival outcomes of 92 breast cancer patients were followed up. Results The positive rates of HIF-2α and Twist2 proteins in the breast carcinoma were higher than those in the fibroadenoma and the adjacent normal tissues (P < 0.05). The high expression of HIF-2α in the breast carcinoma was correlated with tumor size and lymph node metastasis (P < 0.05). The high expression of Twist2 in the breast carcinoma was correlated with tumor grade, lymph node metastasis and ER level (P < 0.05). HIF-2α expression was positively correlated with Twist2 expression in the breast carcinoma (P < 0.05). The 5-year overall survival rates of the patients with HIF-2α-positive and Twist2-positive breast carcinoma were lower than those of the HIF-2α-negative and Twist2-negative patients (P < 0.05). Conclusions HIF-2α and Twist2 are highly expressed in breast carcinoma, both may play promoting roles in tumor invasion and metastasis and affect prognosis. HIF-2α over-expression means poor prognosis, this may be one of the independent factors for predicting the prognosis of the patients with breast cancer.