支气管哮喘患者血清细胞因子、NLRP3 mRNA 及诱导痰黏蛋白水平的变化

Changes of serum cytokines, NLRP3 mRNA and induced sputum mucin in patients with bronchial asthma

摘要:

目的 探讨支气管哮喘患者外周血中单个核细胞Nod 样受体热蛋白结构域相关蛋白3（NLRP3） mRNA、血清白细胞介素-2（IL-2）、IL-6、肿瘤坏死因子-α（TNF-α）、诱导痰黏蛋白水平的变化及 其意义。方法 选取2013 年1 月-2014 年12 月在该院确诊的80 例支气管哮喘患者作为哮喘组，同期收集 健康自愿者80 例作为对照组。检测两组患者外周血NLRP3 mRNA，以及血清IL-2、IL-6、TNF-α、诱 导痰黏蛋白MUC5AC 水平，并进行分析。结果 哮喘组患者NLRP3 mRNA，以及血清IL-6、TNF-α、 MUC5AC 水平高于对照组（P <0.05）；哮喘组患者IL-2 水平低于对照组（P <0.05）；急性发作期哮喘组患 者NLRP3 mRNA，以及血清IL-6、TNF-α、MUC5AC 水平高于慢性持续期哮喘患者（P <0.05）；急性发 作期哮喘组患者IL-2 水平低于慢性持续期哮喘患者（P <0.05）。哮喘组患者治疗后NLRP3 mRNA，以及血 清IL-6、TNF-α、MUC5AC 水平较治疗前降低（P <0.05）；哮喘组患者治疗后IL-2 水平高于治疗前（P <0.05）。 结论 哮喘患者NLRP3 mRNA 及血清IL-6、TNF-α、MUC5AC 水平升高，IL-2 水平降低，并且与患者 病情变化有关。

Abstract:

Objective To investigate the expression of NOD-like receptor heat protein domain-related protein 3 (NLRP3) mRNA in peripheral blood mononuclear cells, and serum levels of interleukin 2 (IL-2), tumor necrosis factor-α (TNF-α) and induced sputum mucin in the patients with bronchial asthma and their significance. Methods Eighty patients with bronchial asthma (asthma group) and 80 healthy volunteers (control group) diagnosed in our hospital from January 2013 to December 2014 were selected. The levels of NLRP3 mRNA, IL-2, IL-6, TNF-α, induced sputum mucin MUC5AC were compared and analyzed. Results The level of NLRP3 mRNA, and the serum levels of IL-6, TNF-α and MUC5AC in the asthmatic group were significantly higher than those in the control group (P < 0.05); the level of IL-2 in the asthmatic group was significantly lower than that in the control group (P < 0.05). The levels of NLRP3 mRNA, serum IL-6, TNF-α and MUC5AC in the patients with acute asthma were significantly higher than those in the patients with chronic persistent asthma (P < 0.05); the level of IL-2 in the patients with acute asthma was significantly lower than that in the patients with chronic persistent asthma (P < 0.05). In the asthma group, the levels of NLRP3 mRNA, serum IL-6, TNF-α, and MUC5AC after treatment decreased significantly compared to those before treatment (P < 0.05); the level of IL-2 after treatment was significantly higher than that before treatment (P < 0.05). Conclusions The levels of NLRP3 mRNA, serum IL-6, TNF-α and MUC5AC increase and IL-2 decreases in asthmatic patients, and these indicators are correlated with the patients’ condition.