葛黄颗粒对酒精性肝损伤大鼠醇、醛脱氢酶 及细胞色素P450 2E1 活性的影响

Protective effect of Ge Huang Granule on liver function in rat model of alcoholic liver injury

摘要:

目的 观察葛黄颗粒对酒精性肝病大鼠醇脱氢酶（ADH）、醛脱氢酶（ALDH）及细胞色素P450 2E1（CYP450 2E1）活性的影响。方法 白酒灌胃加普通饲料饮食复制酒精性肝病大鼠，复制成功后，白酒灌胃继续， 除模型组外，葛黄颗粒高、中、低剂量干预组同时分别给予不同浓度的葛黄颗粒灌胃，正常组一直采用相同剂 量蒸馏水灌胃，13 周后处死大鼠，行肝组织HE 染色、血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）、 ADH、ALDH 含量检测，Western blot 检测肝CYP450 2E1 蛋白表达。结果 与正常组比较，模型组的肝脏指数、 肝功能指标、ADH 及CYP450 2E1 均升高（P <0.01），而ALDH 却降低（P <0.01）；与模型组比较，不同剂量（高、中、 低）葛黄颗粒干预组均可逆转以上检测指标，且存在一定的量效关系，即高、中剂量组逆转效果较好（P <0.01）。 结论 一定剂量葛黄颗粒可通过调节ADH 等乙醇代谢酶活性延缓或逆转酒精性肝损伤的发生。

Abstract:

Objective To investigate the protective effect of Ge Huang Granule on liver function in rats model of alcoholic liver injury. Methods SD rat model of alcoholic liver injury was established with continuous gavage of alcohol (1.0 ml/100 g/1 d). Rat in treatment groups received low dose (group B), moderate dose (group C), and high dose (group D) of Ge Huang Granule. Rats in control group (group A) was only given distilled water (1.0 ml/100 g/1 d). Rats were sacrificed after 13 weeks of insults and liver tissue was collected. Histological analysis of liver was identified by HEstaining.Serum levels of ALT, AST, ADH and ALDH were measured.Western Blot was performed for protein expression of CYP450 2E1. Results Rats in control group experienced increased levels of ADH, ALT, AST and CYP450 2E1 and decreased levels of ALDH, all of which were reversed by treatment of Ge Huang Granule in a dose dependent manner (P < 0.01). Conclusion Ge Huang Granule exerts hepatic protection in rat model of alcoholic liver injury through modulation of ADH and other metabolizing enzymes.