结肠癌组织中基质金属蛋白酶9、转录因子1 与细丝蛋白A 的表达研究

Expression study of matrix metalloproteinase-9, Ets transcription factor-1 and filament protein Ain colon cancer

摘要:

目的 探讨结肠癌患者组织基质金属蛋白酶9（MMP-9）、Ets 转录因子1（Ets-1）与细丝蛋白A（FLNa） 的表达情况。方法 选取已确诊为结肠癌并行手术治疗的患者24 例，术中采集患者肿瘤组织、癌旁组织及正 常结肠组织，检测各组织中MMP-9、Ets-1 与FLNa 表达水平，并分析肿瘤组织的临床病理指标与MMP-9、 Ets-1 与FLNa 表达水平的关系，MMP-9 与Ets-1、FLNa 表达的相关性。结果 ① MMP-9 与Ets-1 表达水 平比较，肿瘤组织> 癌旁组织> 正常结肠组织（P<0.05）；FLNa 表达水平比较，肿瘤组织< 癌旁组织< 正 常结肠组织（P <0.05）；②肿瘤组织中MMP-9 与Ets-1 高表达（P <0.05），FLNa 低表达（P <0.05），与肿瘤 直径越大、Dukes 分期越严重、分化程度越低、术后有复发转移有关；③在癌旁组织及肿瘤组织中，MMP-9 与Ets-1 的表达呈正相关（P <0.05），与FLNa 表达呈负相关（P <0.05）。结论 MMP-9 与Ets-1 在结肠癌组 织中的表达正相关，与FLNa 表达负相关，MMP-9、Ets-1 表达水平升高，FLNa 表达水平降低。

Abstract:

Objective To investigate the expression levels of matrix metalloproteinase-9 (MMP-9), Ets transcription factor-1 (Ets-1) and filament protein A (FLNa) in colon cancer patients. Methods Totally 24 patients diagnosed with colon cancer were enrolled. Cancer tissues, para-cancerous tissues and normal colorectal tissues were collected. The expression levels of MMP-9, Ets-1 and FLNa were determined.Correlations between tumor clinicopathological parameters and the expression levels of MMP-9, Ets-1and FLNawereanalyzed. Results Expression of MMP-9 and Ets-1 was increased while FLNa was decreased significantly in tumor tissues when compared with paracancerous tissues or normal colon tissues (P < 0.05). Downregulated expression of FLNa and upregulated expression of MMP-9 and Ets-1 were closely correlated with tumor size, Dukes staging, degree of differentiation and the recurrence and metastasis after surgery (P < 0.05). In adjacent tissues and tumor tissues, the expression of MMP-9 was positively correlated with Ets-1 (P < 0.05) while negative relationship was identified between MMP-9and FLNa (P < 0.05). Conclusion Upregulation of MMP-9 and Ets-1 and downregulation of FLNa in colon cancer tissues are closely correlated with recurrence and metastasis of colon cancer.