燕麦β- 葡聚糖对脓毒血症大鼠肠黏膜 损伤的保护作用及其机制探讨

Oat β-glucan alleviates intestinal mucosal injury in rats with sepsis by inhibiting inflammation response

摘要:

目的 探索燕麦β- 葡聚糖（Oglu）能否对脓毒血症空肠损伤提供保护。方法 选取50 只SD 大鼠， 按随机数字表法分为对照组、脓毒血症组、Oglu 组。脓毒血症组和Oglu 组以改良盲肠结扎穿孔法复制脓毒 血症模型，通过病理检查评估空肠损伤。采用酶联免疫吸附法检测炎症因子白细胞介素-6（IL-6），肿瘤坏 死因子-α（TNF-α）和降钙素原，PCR、Western blot 检测低氧诱导因子-1（HIF-1α）、β- 连环蛋白 （β-catenin）、T 细胞因子（TCF-4）、基质金属蛋白酶-13（MMP-13）表达的变化。结果 Oglu 缓解脓毒 血症诱导的空肠损伤，降低脓毒血症大鼠IL-6、TNF-α 及降钙素原水平。Oglu 组空肠组织中HIF-1α 表 达高于对照组和脓毒血症组（P <0.05）；脓毒血症组与对照组比较，差异无统计学意义（P >0.05）；Oglu 组 β-catenin、TCF-4 及MMP-13 表达水平较脓毒血症组降低（P <0.05）。结论 Oglu 对脓毒血症大鼠空肠 损伤发挥保护作用，可能与HIF-1α、Wnt/β-catenin 信号通路调控，减轻脓毒血症期间氧化应激及炎症反 应有关。

Abstract:

Objective To investigate whether oat β-glucan (Oglu) can provide protection for jejunum injury with sepsis. Methods Fifty SD rats were randomly divided into a normal control group (n = 10), a sepsis group (n = 20) and an Oglu group (n = 20). Septic models in the sepsis group and the Oglu group were induced with cecal ligation and puncture (CLP). The jejunum injury was assessed by pathology. And the inflammatory cytokines IL-6, TNF-α and procalcitonin were detected by ELISA. The expressions of hypoxia-inducible factor-1 (HIF-1α), β-catenin, T-cell factor (TCF-4) and matrix metalloproteinase 13 (MMP-13) were detected by PCR and Western blot. Results Oglu relieved sepsis-induced jejunum injury, and reduced the levels of IL-6, TNF-α and procalcitonin in the sepsis group (P < 0.05). The expression of HIF-1α in the jejunum of the Oglu group was higher than that of the normal control group and the sepsis group (P < 0.05), but there was no significant difference between the latter two groups (P > 0.05). The expressions of β-catenin, TCF-4 and MMP-13 in the Oglu group was significantly lower than that in the sepsis group (P < 0.05). Conclusions Oat β-glucan exerts protective effect on jejunum in septic rats, which is related to the regulation of HIF-1α and Wnt/β-catenin signaling pathway and the reduction of oxidative stress and inflammatory response during sepsis.