布地奈德对哮喘小鼠IL-17 表达的影响

Effect of Budesonide on IL-17 expression level in asthmatic mice

摘要:

目的 探讨白细胞介素17（IL-17）在哮喘小鼠发病机制中的作用，布地奈德控制哮喘的作用机 制与IL-17 表达的关系。方法 24 只4 周龄清洁级雌性BALB/c 小鼠，随机分为正常组、哮喘组及布地奈德组， 每组8 只。以卵清蛋白（OVA）致敏、激发复制哮喘小鼠模型。采用雾化吸入的方法给予哮喘小鼠布地奈德 混悬液治疗。模型复制成功后，取其肺组织行病理切片观察肺组织病理变化，并行实时荧光定量聚合酶链反 应（qRT-PCR），测定肺组织中IL-17 mRNA 的表达。取支气管肺泡灌洗液（BALF）行酶联免疫吸附试验 （ELISA）检测IL-17 细胞因子的水平。结果 哮喘组气道中炎症细胞浸润、BALF 中IL-17 表达、肺组织中 IL-17 mRNA 表达与正常组比较，差异有统计学意义（P <0.05），哮喘组均增高。经布地奈德雾化吸入干预后， 布地奈德组气道中炎症细胞、BALF 中IL-17 的表达、肺组织中IL-17 mRNA 表达与哮喘组比较，差异有统 计学意义（P <0.05），布地奈德组低于哮喘组。结论 IL-17 在哮喘小鼠中的表达升高，抑制小鼠中IL-17 的表达， 是布地奈德控制哮喘的作用机制之一。

Abstract:

Objective To explore the role of IL-17 in the pathogenesis of asthmatic mice, and the relationship between the action mechanism of Budesonide controlling asthma and the expression level of IL-17. Methods Twenty-four female BALB/c mice at the age of 4 weeks were randomly divided into a control group, an asthma group and a Budesonide group, and each group had 8 mice. The mouse model of asthma was made by sensitization with ovalbumin (OVA). The asthmatic mice were treated with Budesonide suspension by atomization inhalation. After the success of the model, the pathological changes of the lung tissues were observed, and the expression level of IL-17 mRNA in the lung tissues was determined by qRT-PCR. The IL-17 level in the bronchoalveolar lavage fluid (BALF) was detected by ELISA. Results Compared with the control group, inflammatory cell infiltration in the airway of the asthma group and the expression level of IL-17 in BALF and IL-17 mRNA in the lung tissues were significantly higher (P < 0.05). After intervention with Budesonide atomization inhalation, the inflammatory cell infiltration in the airway and the expression level of IL-17 in BALF and IL-17 mRNA in the lung tissues in the Budesonide group were lower than those in the asthma group (P < 0.05). Conclusions The expression level of IL-17 is elevated in asthmtic mice. Inhibiting the expression level of IL-17 in mice is one of the mechanisms of Budesonide for asthma control.