烟雾对支气管哮喘大鼠肺组织激活素A 表达的影响

Impact of cigarette smoke exposure on expression of activin A in lung tissue of OVA-sensitized asthmatic rats

摘要:

目的 观察烟雾暴露对卵白蛋白（OVA）致敏的急性支气管哮喘（以下简称哮喘）大鼠模 型肺组织中激活素A（ActA）表达的变化，探讨烟雾对哮喘的影响。方法 雄性SD 大鼠32 只，随机分为 4 组：正常对照组、烟雾对照组、哮喘模型组及烟雾哮喘组，分别复制哮喘和烟雾暴露模型。采用苏木精- 伊红染色观察肺组织病理学改变，实时荧光定量聚合酶链反应和Western blot 检测各组大鼠肺组织中ActA mRNA 和蛋白表达的变化。结果 ①哮喘模型组和烟雾对照组ActA mRNA 表达水平高于正常对照组 （P <0.05），烟雾哮喘组ActA mRNA 表达水平高于哮喘模型组和烟雾对照组（P <0.05）；②哮喘模型组和烟 雾对照组ActA 蛋白表达水平高于正常对照组（P <0.05），烟雾哮喘组ActA 蛋白表达水平高于哮喘模型组和 烟雾对照组（P <0.05）。结论 烟雾暴露可以提高OVA 致敏的急性哮喘大鼠模型肺组织中ActA mRNA 表达 水平，促进ActA 蛋白的表达，从而加重气道炎症。

Abstract:

Objective To observe the change of activin A (ActA) expression in the lung tissue of ovalbumin (OVA)-sensitized asthmatic rats after exposure to smoke, and explore the impact of smoke on asthma. Methods Thirty-two male Sprague-Dawley rats were randomly divided into four groups with 8 rats in each group, namely a normal control group, an asthmatic model group, a cigarette smoke control group, a cigarette smoke exposure and asthmatic group. The rats in the exposed groups were exposed to either aerosolized OVA, tobacco smoke, or both tobacco smoke and OVA, respectively. The pathological changes of the lung tissue were observed after HE staining. The expressions of ActA mRNA and protein in the lung tissue were detected by qRT-PCR and Western blot (WB). Results The levels of ActA mRNA in the asthmatic model group and the smoke control group were significantly higher than that in the normal control group (P < 0.05), and the level of ActA mRNA in the smoke exposure and asthmatic group was significantly higher than those in the asthmatic model group and the smoke control group (P < 0.05). The levels of ActA protein in the asthmatic model group and the smoke control group were significantly higher than that in the normal control group (P < 0.05), and the level of ActA mRNA in the smoke exposure and asthmatic group was significantly higher than those in the asthmatic model group and the smoke control group (P < 0.05). Conclusions Cigarette smoke can increase the mRNA and protein expression levels of ActA in the lung tissue of OVA-sensitized asthmatic rats, and exacerbate the asthmatic airway inflammation.