三溴丙酮酸对MCF-7 细胞增殖、侵袭的 影响及其机制研究

Effect of tribromopyruvic acid on proliferation and invasion of MCF-7 cells and its mechanism

摘要:

目的 探讨三溴丙酮酸（3-BrPA）对MCF-7 细胞增殖、侵袭，以及对真核转录起始因子5A2 （eIF5A2）、c-myc 基因、转移相关蛋白1（MTA1）表达的影响。方法 实验分为5 组：加入等量PBS 的 对照组，75、100、125 和150μmol/L 3-BrPA 组。分别将3-BrPA 作用于MCF-7 细胞24、48 和72 h，利 用MTT、Transwell 法检测3-BrPA 对MCF-7 细胞存活率和侵袭的影响。采用逆转录聚合酶链反应（RTPCR） 和Western blot 检测eIF5A2、c-myc、MTA1 基因和蛋白的变化。结果 与对照组比较，3-BrPA 作 用于乳腺癌MCF-7 细胞后，细胞存活率降低（P <0.05），并且具有时间和剂量依赖性。Transwell 结果显 示，3-BrPA 作用于MCF-7 细胞24 h 后，与对照组相比，穿过小室的细胞数减少（P <0.05）。RT-PCR 和 Western blot 检测结果提示，eIF5A2、c-myc、MTA1 基因和蛋白表达水平随着药物浓度的增加而降低（P <0.05）。 结论 3-BrPA 可以抑制乳腺癌MCF-7 细胞的增殖及侵袭，其作用机制可能与降低eIF5A2、c-myc、MTA1 基因和蛋白的表达有关。

Abstract:

Objective To investigate the effect of tribromopyruvic acid (3-BrPA) on the proliferation and invasion of breast cancer MCF-7 cells and the expressions of eukaryotic initiation factor 5A2 (eIF5A2 ), c-myc and metastasis-associated protein 1 (MTA1). Methods There were 5 experimental groups, namely control group (adding PBS), 75 μmol/L 3-BrPA group, 100 μmol/L 3-BrPA group, 125 μmol/L 3-BrPA group and 150 μmol/L 3-BrPA group. MTT and Transwell assay were used to detect the effect of 3-BrPA on the viability and invasion of MCF-7 cells after treated with 3-BrPA for 24, 48 and 72 h respectively. RT-PCR and Western blot were used to detect the changes of eIF5A2 , c-myc and MTA1 genes and proteins. Results Compared with the control group, the viability of the MCF-7 cells treated with 3-BrPA was significantly decreased (P < 0.05) in a time- and dose-dependent manner. Transwell results showed that the number of MCF-7 cells passing through the wells was significantly reduced after 3-BrPA interaction for 24 h (P < 0.05) compared to the control group. RT-PCR and Western blot showed that the expression levels of eIF5A2 , c-myc and MTA1 genes and proteins decreased with the increase of drug concentration (P < 0.05). Conclusions Tribromopyruvic acid can inhibit the proliferation and invasion of breast cancer MCF-7 cells. The mechanism may be related to the decrease of eIF5A2 , c-myc and MTA1 gene and protein expressions.