绝经后女性2 型糖尿病患者的DKK1 蛋白 表达水平及其临床意义

Expression level of DKK1 protein in postmenopausal women with type 2 diabetes mellitus

摘要:

目的 探讨Wnt 信号通路中DKK1 蛋白在绝经后2 型糖尿病（T2DM）女性患者中的表达及其 与胰岛素抵抗和胰岛β 细胞功能的关系。方法 选取2015 年10 月-2017 年10 月于石河子大学第一附属医 院和新疆维吾尔自治区石河子市第十五社区卫生服务中心住院的绝经后女性T2DM 患者。收集患者临床资 料并检测常规生化指标、空腹胰岛素（FINS）、空腹C 肽（FCP）及DKK1 蛋白表达水平，计算胰岛素敏感 指数（ISI）、胰岛素抵抗指数（HOMA-IR）及胰岛素β 细胞功能指数（HOMA-β）。采用SPSS 20.0 统计 软件进行数据分析。结果 与NGT 组相比，T2DM 组FPG、果糖胺、HbA1c、TC 及HOMA-IR 水平提高， FCP、ISI 及HOMA-β 水平降低（P <0.05）。T2DM 组DKK1 蛋白表达水平升高（P <0.05）。DKK1 蛋白 表达水平与FCP 呈负相关，与HOMA-IR 呈正相关（P <0.05）。FPG、DKK1 蛋白是影响FCP 及HOMAIR 水平的因素（P <0.05）。结论 Wnt 信号通路中DKK1 蛋白水平的高表达可能参与绝经后女性T2DM 患者胰岛素抵抗、胰岛β 细胞功能减退的发生发展过程，进而导致绝经后女性T2DM 的糖代谢及脂代谢 紊乱。

Abstract:

Objective To study the expression of DKK1 protein of Wnt signaling pathway, and analyze the relationship of DKK1 expression with insulin resistance and islet beta cell function in postmenopausal women with type 2 diabetes mellitus (T2DM). Methods Postmenopausal women in the First Affiliated Hospital of Shihezi University and the 15th Community Health Service Center of Shihezi City in Xinjiang Uygur Autonomous Region between October 2015 and October 2017 were collected into a NGT group and a T2DM group. The general clinical data and biological indicators were recorded. The levels of conventional biochemical indicators and fasting insulin and fasting c-peptide (FCP) as well as the expression level of DKK1 protein were measured. Insulin sensitivity index (ISI), insulin resistance index (HOMA-IR) and insulin β cell function index (HOMA-β) were calculated. Data was analyzed by SPSS 20. 0. Results Compared with the NGT group, FPG, FMN, HbA1c, TC and HOMA-IR increased; however, FCP, ISI and HOMA-β decreased in the T2DM group (P < 0.05). The expression level of DKK1 protein increased in the T2DM group (P < 0.05). The serum level of DKK1 protein was negatively correlated with FCP, but positively correlated with HOMA-IR in postmenopausal women with T2DM (P < 0.05). FPG and DKK1 protein were the factors influencing FCP and HOMA-IR levels (P < 0.05). Conclusions The higher expression of DKK1 protein in Wnt signaling pathway may be involved in the development process of insulin resistance and decreasing islet cell function, thereby leading to the disorders of carbonhydrate and lipid metabolisms in postmenopausal women with T2DM.