五味子酚对心肌成纤维细胞增殖和转化生长因子 β1 及Ⅰ、Ⅲ型胶原蛋白表达的影响

Effect of Schisanhenol on proliferation of cardiac fibroblasts and potential mechanisms

摘要:

目的 研究五味子酚（Sal）对血管紧张素Ⅱ（Ang Ⅱ）诱导的心肌成纤维细胞（CFb）增殖及转 化生长因子β1（TGF-β1）和Ⅰ、Ⅲ型胶原蛋白表达的影响，探讨Sal 抗心肌纤维化的可能机制。方法 采用 酶消化法结合差速贴壁法获得纯化的CFb ；利用免疫荧光法鉴定CFb ；采用MTT 法检测Sal 对CFb 增殖的 影响；采用Western blot 检测CFb 的TGF-β1 及Ⅰ、Ⅲ型胶原蛋白表达水平。结果 在一定浓度范围内，Sal 能抑制Ang Ⅱ诱导的CFb 增殖且具有剂量依赖性（P <0.05）；Sal 能抑制TGF-β1 及Ⅰ、Ⅲ型胶原蛋白表达， 与Ang Ⅱ组比较差异有统计学意义（P <0.05），且具有剂量依赖性。结论 Sal 能抑制CFb 增殖及降低Ⅰ、Ⅲ 型胶原蛋白表达，其机制可能与下调TGF-β1 蛋白表达有关。

Abstract:

Objective To investigate the effect of Schisanhenol (Sal) on proliferation of cardiac fibroblasts (CFb) and potential mechanisms. Methods Purified CFb were obtained by standard methods (digestive enzyme and differential adhesion). CFb were induced by angiotensin II (Ang II) and identified by immunofluorescence. Cellular proliferation of CFb was measured by methyl thiazolyl tetrazolium (MTT) assay. Levels of TGF-β1, collagen I and collagen III proteins were examined by Western blotting. Results Sal inhibited proliferation of CFb in a dose-dependent manner (P < 0.05). Expression of collage I, collagen III, and TGF-β1 induced by Ang II was downregulated by treatment of Sal in a dose-dependent manner (P < 0.05). Conclusion Sal inhibits cellular proliferation and expression of collagen through inhibition of the TGF-β1.