选择性脊髓麻醉动物模型的复制

Selectively-anesthetized spinal cord model in New Zealand white rabbits

摘要:

目的 利用乳化七氟烷独特药代动力学特点，用新西兰大白兔复制一种小动物选择性脊髓麻醉模型。 方法 选取16 只雄性新西兰大白兔，分为动脉组和静脉组。动脉组经腹主动脉（T12 ～ L1）导管按8 ml/（kg·h） 持续泵入8% 乳化七氟烷；静脉组经右侧耳缘静脉泵入相同剂量的乳化七氟烷。气相色谱仪和顶空二次平衡 法测量颈动脉、颈静脉（代表脑）和股动脉、股静脉（代表脊髓）血液中七氟烷浓度和分压。结果 静脉组 动物不同部位血液中七氟烷浓度、分压比较差异无统计学意义（P >0.05）。动脉组颈动脉和颈静脉，股动脉 和股静脉中七氟烷浓度、分压比较差异无统计学意义（P >0.05）；动脉组股动脉和颈动脉，股静脉和颈静脉 中七氟烷分压比较差异有统计学意义（P <0.05）。股动脉七氟烷分压是颈动脉的（3.26±0.37）倍；股静脉七 氟烷分压是颈静脉的（3.87±0.85）倍。结论 在保留新西兰大白兔脑和脊髓正常血液循环结构基础上，利用 新西兰大白兔节段性脊髓血供特点和乳化七氟烷独特药代动力学模型，成功复制了一种小动物选择性脊髓（腰 骶段）麻醉模型。

Abstract:

Objective To develop a new selectively-anesthetized spinal cord model in New Zealand white rabbits by emulsified Sevoflurane. Methods Sixteen New Zealand white rabbits were randomly divided into two groups. In the group vein, 8% emulsified Sevoflurane was infused via the ear vein at 8 ml/(kg·h). In the group artery, 8% emulsified Sevoflurane was infused into abdominal aorta (T12-L1) by catheter at 8 ml/(kg·h). The concentration (Csevo) and partial pressure (Psevo) of Sevoflurane in carotid artery and jugular vein (representing the brain) and femoral artery and vein (representing the spinal cord) were determined using a gas chromatograph with the two-stage head space equilibrium method. Results In the group vein, there were no significant differences in Csevo or Psevo among different sites (P > 0.05). In the group artery, there was no significant difference in Csevo or Psevo between carotid artery and jugular vein, or between femoral artery and vein (P > 0.05). However, the Psevo of the femoral artery was (3.26 ± 0.37) times that of the carotid artery, the Psevo of the femoral vein was (3.87 ± 0.85) times that of the jugular vein, there were significant differences (P < 0.05). Conclusions Based on the segmental spinal cord circulation system of New Zealand white rabbits, we successfully established selectively-anesthetized spinal cord (lumbar and sacro-coccygeal region) model by emulsified Sevoflurane.