薯蓣素抑制口腔鳞状细胞癌的机制研究

Inhibitory effect of Dioscin on oral squamous cell carcinoma

摘要:

目的 研究薯蓣素对人口腔鳞状细胞癌的抑制作用。方法 薯蓣素处理TSCCa 和TCa8113 细胞，MTS 和软琼脂集落实验检测薯蓣素对口腔鳞状细胞癌增殖的影响。蛋白质免疫印迹检测薯蓣素对口腔鳞状细胞癌 糖酵解调控蛋白及凋亡调控分子表达的影响。检测过表达己糖激酶HK2 对薯蓣素诱导细胞凋亡的作用。结果 薯蓣素抑制TSCCa 和TCa8113 细胞的增殖具有剂量和时间依赖性（P <0.05）。薯蓣素抑制口腔鳞状细胞癌 糖酵解（P <0.05），并下调HK2 和葡萄糖转运蛋白Glut1 的表达。薯蓣素能诱导口腔鳞状细胞癌Caspase-3 和 PARP 剪切体表达上调，过表达HK2 抑制薯蓣素诱导的细胞凋亡。结论 薯蓣素对口腔鳞状细胞癌的抑制作用 与其下调HK2 和对糖酵解的抑制有关。

Abstract:

Objective To investigate the inhibitory effect of Doscin on human oral squamous cell carcinoma (OSCC). Methods TSCCa and TCa8113 oral squamous carcinoma cell line were treated with various concentration of Dioscin. Cell proliferation was tested via MTS and soft agar colony formation assay. Cell glycolysis was analyzed. Glycolysis and apoptosis related proteins were measured via immunoblotting assay. Western blot was performed to determine the effect of hexokinase-2 (HK2) overexpression on Dioscin-induced cellular apoptosis. Results Dioscin decreased the cell growth of TSCCa and TCa8113 cells in a dose- and time- dependent manner (P < 0.05). Dioscin significantly suppressed glycolysis in TSCCa and TCa8113 cells and decreased the protein levels of Glut1 and HK2 (P < 0.05). Additionally, Dioscin elevated the expression of cleaved-caspase-3 and cleaved-PARP. Overexpression of HK2 inhibited Dioscin-induced cell apoptosis. Conclusions Our results suggests that Dioscin inhibits OSCC growth through inhibition of HK2 and glycolysis.