miRNA-135b 对鼻咽癌细胞侵袭 和转移的促进作用

miRNA-135b promotes invasion and metastasis of nasopharyngeal carcinoma cells

摘要:

目的 研究miRNA-135b 对鼻咽癌CNE1 细胞系侵袭和转移的影响和作用机制。方法 用逆转录聚 合酶链反应（RT-PCR）检测miRNA-135b 在鼻咽癌CNE1 细胞系中的表达，分别在鼻咽癌CNE1 细胞系中 过表达和下调miRNA-135b，通过微孔滤膜培养小室及双室联合培养系统（Transwell TM）检测肿瘤细胞迁移和 侵袭能力，采用Western blotting 检测大型肿瘤抑制因子同源蛋白2（LATS2）的表达。结果 miRNA-135b 在 鼻咽癌CNE1 细胞系中高表达，通过增强miRNA-135b 表达，鼻咽癌CNE1 细胞的侵袭和转移能力有所提升； 通过靶向miRNA-135b 干扰载体，下调miRNA-135b 表达，鼻咽癌CNE1 细胞的侵袭和转移能力有所下降。 增强miRNA-135b 表达，LATS2 活性和表达下降，而下调miRNA-135b 表达，LATS2 活性和表达上升。结 论 miRNA-135b 过表达会提升鼻咽癌CNE1 细胞的侵袭和转移能力，下调miRNA-135b 表达可有效抑制鼻 咽癌细胞增殖，促进细胞凋亡。miRNA-135b 的表达可能通过影响LATS2 活性影响鼻咽癌细胞的生长。

Abstract:

Objective To investigate the effect of miRNA-135b in invasion and metastasis of nasopharyngeal carcinoma (NPC) and potential mechanisms. Methods Expression of miRNA-135b in NPC tissues and NPC cell lines were detected by RT-PCR. The invasion and metastasis were identified by Transwell TM assay. Expression of LATS2 protein and activity of LATS2 protein were determined by western blot and LATS2 activity assay kit, respectively. Results Expression of miRNA-135b was significantly increased in NPC cell lines. Transwell TM assay showed that over-expression of miRNA-135b induced a dramatic improvement of invasion and metastasis in CNE1 cells, which was reversed by knockdown of miRNA-135b. Expression and activity of LATS2 were decreased significantly by over-expression of miRNA-135b, which was abolished by knockdown of miRNA-135. Conclusions miRNA-135b promotes the invasion and metastasis of nasopharyngeal carcinoma cells through mediating activity of LATS2.