尼古丁对A549 化学治疗敏感性的影响

Effect of Nicotine on chemo-sensitivity in A549

摘要:

目的 探讨尼古丁（Nicotine）诱导的肺癌A549 细胞对顺铂的敏感性，以及对凋亡相关蛋白表达水 平的影响。方法 将A549 细胞随机分为对照组、顺铂（CDDP）组和CDDP+Nicotine 组，培养48 h，MTT 法检 测细胞活力，流式细胞术检测细胞凋亡情况，Western blotting 检测凋亡相关蛋白Bcl-2、Bak 和Mcl-1 的表达 水平。结果 与CDDP 组比较，CDDP+Nicotine 组的细胞活力更高，细胞凋亡率更低，促凋亡蛋白Bak 的表 达无变化，抗凋亡蛋白Bcl-2 和Mcl-1 的表达上调，且Mcl-1 磷酸化水平上调。结论 尼古丁可能通过上调 Bcl-2 和Mcl-1 的表达，以及Mcl-1 的磷酸化水平降低肺癌细胞A549 对CDDP 的敏感性。

Abstract:

Objective To investigate the sensitivity of lung cancer cell A549 induced by Nicotine to Cisplatin (CDDP) and its effect on apoptosis-related protein. Methods A549 cells were randomly divided into control group, CDDP group and CDDP + Nicotine group. After co-cultured for 48 hours, cell viability was measured by MTT assay, apoptosis was detected by flow cytometry, and expression levels of apoptosis-related protein Bcl-2, Bak and Mcl-1 were identified by western blot. Results Compared with the CDDP group, the cell viability was higher and apoptosis rate was lower in Cisplatin + Nicotine group while no significant change in pro-apoptotic protein Bak was identified. Expression of anti-apoptotic proteins Bcl-2 and Mcl-1 and phosphorylation level of Mcl-1 were upregulated when compared with those in CDDP group. Conclusions Nicotine may reduce the sensitivity of lung cancer cell A549 to CDDP by up-regulating the expression of Bcl-2 and Mcl-1, and the phosphorylation of Mcl-1.