血清半乳凝集素3 介导心房颤动机制的相关研究

Role of Galectin-3 in atrial fibrillation

摘要:

目的 研究心房颤动（AF）患者血清半乳凝集素3（Gal-3）水平与心肌纤维化指标的关系，探 讨Gal-3 介导心房颤动的机制。方法 选取盛京医院心血管内科住院心功能正常的40 例房颤患者作为房颤 组，同期在选取本院20 例健康体检者作为对照组。采用酶联免疫吸附法（ELISA）检测患者血清中Gal-3、 转化生长因子β1（TGF-β1）、Smad3 蛋白及I 型前胶原羧基端肽（PICP）浓度；比较两组各指标差异，并 分别对Gal-3 与其他指标进行相关性分析。结果 与对照组比较，房颤组Gal-3、TGF-β1、Smad3、PICP 增 高（P <0.05）；房颤组Gal-3 与PICP、Gal-3 与TGF-β1、TGF-β1 与Smad3 及TGF-β1 与PICP 均呈正相 关（r =0.695、0.760、0.890 及0.829，均P =0.001）。结论 房颤患者血清Gal-3 及心肌纤维化指标水平增高， Gal-3 可通过加速TGF-β1/Smad3 信号通路的传导促进心肌纤维化，最终导致心房颤动的发生。

Abstract:

Objective To study the relationship between Galectin-3 (Gal-3) and atrial fibrillation (AF) in patients with atrial fibrillation and potential mechanisms. Methods A total of 40 AF patients who had normal cardiac function were selected as AF group, and 20 healthy subjects were enrolled as control group. Levels of Gal-3, transforming growth factor-beta 1, Smads protein and precollagen type I carboxy terminal peptide were measured by enzyme-linked immunosorbent assay. Correlation between Gal-3 and other parameters was analyzed. Results Levels of Gal-3, TGF-β1, Smad3 and PICP in AF group were increased significantly compared with those in control group (P < 0.05). Statistical analysis showed that there was a significant positive correlation between Gal-3 and PICP, Gal- 3 and TGF-beta 1, TGF-beta 1 and smad3, and TGF-beta 1 and PICP in AF group (r = 0.695, 0.760, 0.890 and 0.829; all P = 0.001). Conclusions Gal-3 and myocardial fibrosis indices in AF patients increase and Gal-3 may promote myocardial fibrosis by activating TGF- beta 1/Smad3 signal pathway.