肝癌中骨形态发生蛋白7 表达 及其与生存相关性分析

Expression of BMP-7 in hepatocellular carcinoma and its relationship with clinical prognosis

摘要:

目的 研究肝癌组织中骨形态发生蛋白7（BMP-7）表达及其与患者预后关系。方法 免疫组织化 学及实时逆转录聚合酶链反应（real-time RT-PCR）方法分析肝癌组织、癌旁组织及非癌肝脏组织中BMP-7 表达，选取2010 年1 月—2013 年12 月江苏省连云港市第一人民医院85 例肝癌患者的癌组织及20 例癌旁组 织标本，以肝血管瘤及肝脏外伤患者14 例为良性对照。研究BMP-7 与肝癌患者临床病理特征之间关系及其 不同表达患者术后生存情况。结果 BMP-7 在肝癌组织、癌旁组织及非癌肝脏组织中表达的阳性率比较，差 异有统计学意义（P <0.05），BMP-7 的表达与患者肿瘤大小、肿瘤组织分化程度相关，其阳性表达的肝癌患 者预后较阴性表达者差。结论 BMP-7 与肝癌的发生、发展相关，可以作为预测患者预后情况的重要指标。

Abstract:

Objective To study the expression of BMP-7 in hepatocellular carcinoma (HCC) and its relationship with clinical prognosis. Methods A total of 85 patients with HCC admitted into our hospital from January 2010 to December 2013 were enrolled in this study. Twerty cases of pura -carcinoma tissnes and 14 cases of hepatic hemangioma or hepatic injury tissues were also collectd. Expression of BMP-7 in HCC tissues (n = 85), adjacent tissues (n = 20), and non-cancerous liver tissues (n = 14) was analyzed by immunohistochemistry and RT-PCR. Relationship between BMP-7 and clinicopathological features was investigated. Results The positive expression rate of BMP-7 in hepatocellular carcinoma, adjacent tissues and non-cancerous liver tissue was significantly different. The expression of BMP-7 was closely correlated to the tumor size and differentiation degree of tumor tissue. Patients with positive expression of BMP-7 experienced poor outcome. Conclusion BMP-7 may play an important role in the carcinogenesis and progression of HCC, which may be an important prognostic indicator.