子宫内膜癌组织microRNA-944 的表达及 临床意义

Role of microRNA-944 in patients with endometrial carcinoma

摘要:

目的 探讨子宫内膜癌组织microRNA-944（miR-944）的表达及其与子宫内膜癌（EC）患者临 床病理特征及预后的关系，并分析miR-944 对患者的早期诊断价值。方法 采用实时荧光定量聚合酶链反应 （qRT-PCR）检测EC 癌组织（80 例）和正常子宫内膜组织（30 例）中miR-944 的表达情况，分析miR-944 表达与EC 患者临床病理特征之间的关系。采用Kaplan-Meier 生存曲线法分析miR-944 表达与EC 患者预后的 关系，并采用受试者操作特征曲线（ROC）分析miR-944 对EC 的早期诊断价值。结果 qRT-PCR 结果显示， miR-944 在EC 癌组织中的表达水平高于正常子宫内膜组织（P <0.05）。在EC 癌组织中，miR-944 表达水平与 肿瘤肌层浸润深度、有无淋巴转移、有无远处转移及国际妇产联盟（FIGO）分期相关（P <0.05），而与患者年 龄、绝经状态、病理分型和组织分级无关（P >0.05）。Log-rank 检验结果显示，miR-944 高表达组患者的生存 时间较miR-944 低表达组降低（P <0.05）。ROC 分析结果显示，miR-944 的曲线下面积（AUC）为0.784。结论 EC 癌组织中miR-944 呈高表达，且其高表达与EC 患者不良预后密切相关，miR-944 可作为EC 早期诊断和预 后预测的潜在靶点。

Abstract:

Objective To investigate expression of microRNA-944 (miR-944) in patients with endometrial carcinoma (EC), and to determine its clinical significance. Methods The expression level of miR-944 in 80 cases of EC cancer tissues and 30 cases of normal endometrial tissues were measured by real-time quantitative fluorescence PCR (real-time PCR) test. Relationship between the expression of miR-944 and the clinical characteristics of patients with EC was analyzed. Results RT-PCR showed that the expression level of miR-944 in EC cancer tissue was significantly higher than that in normal endometrium (P < 0.05). Expression of miR-944 was correlated to the depth of tumor myometrium infiltration, lymph node metastasis, distant metastasis and FIGO stage (P < 0.05). Log-rank test showed that the survival time of the patients with high expression of miR-944 was decreased obviously compared with that in patients with low expression group of miR-944 (P < 0.05). ROC analysis showed that the area under the curve of miR-944 (AUC) was 0.784. No obvious correlation was identified between miR-944 and age, menopausal status, pathological classification, or histological grade (P > 0.05). Conclusions High expression of miR-944 in EC cancer tissue relates to the poor prognosis, which may be used as a potential diagnostic and prognostic biomarker.