LncRNA-ATB 在非小细胞肺癌细胞中的 作用及其机制探讨

LncRNA-ATB promotes migration and invasion of NSCLC cells by competitive binding with miR-141

摘要:

目的 检测被转化因子激活的长链非编码RNA（LncRNA-ATB）在非小细胞肺癌（NSCLC） 细胞中的表达，及沉默LncRNA-ATB 对NSCLC 细胞侵袭和转移能力的影响。方法 采用实时荧光定量 聚合酶链反应（qRT-PCR）检测NSCLC 细胞和正常支气管上皮细胞（HBEC）中LncRNA-ATB 的表达 水平；采用细胞划痕实验和Transwell 实验检测沉默LncRNA-ATB 对NSCLC 细胞侵袭和转移的影响；采 用荧光素酶报告基因实验和qRT-PCR 检测LncRNA-ATB 与microRNA-141（miR-141）的调控关系。 结果 LncRNA-ATB 在NSCLC 细胞中的表达高于HBEC 细胞（P <0.05）。沉默NSCLC 中LncRNAATB 表达后，细胞侵袭和转移能力降低。LncRNA-ATB 可竞争性结合miR-141，并下调其表达。结论 LncRNA-ATB 在NSCLC 中表达上调，并可通过竞争性结合miR-141，促进NSCLC 的侵袭和转移。

Abstract:

Objective To explore the expression of LncRNA-ATB in non-small cell lung cancer (NSCLC), and investigate the effect and underlying mechanism of LncRNA-ATB on migration and invasion of NSCLC cells. Methods Expression of LncRNA-ATB in NSCLC cells and normal bronchial epithelial cells was analyzed by qRT-PCR. Effect of LncRNA-ATB on migration and invasion of NSCLC cells was detected by Transwell assay and wound healing assay. Luciferase assay was performed to detect whether LncRNA-ATB could sequester miR-141. qRT-PCR was performed to detect the relationship between LncRNA-ATB and miR-141 in NSCLC cells. Results LncRNA-ATB was up-regulated in the NSCLC cells compared to the normal bronchial epithelial cells (P < 0.05). Migration and invasion of NSCLC cells were suppressed after silencing LncRNA-ATB. LncRNA-ATB could directly bind to miR-141 and down-regulate its expression in NSCLC cells. Conclusions LncRNA-ATB is upregulated in NSCLC cells, and could promote migration and invasion of NSCLC cells by competitive binding to miR- 141.