黑色素瘤中细胞凋亡抑制蛋白Survivin 和MEF2D 的表达及意义

Expressions of apoptosis inhibitor proteins Survivin and MEF2D in melanoma and significance

摘要:

目的 探究凋亡抑制蛋白Survivin 和MEF2D 在黑色素瘤中的表达及其相关性。方法 临床收 集21 例黑色素瘤组织和10 例皮内痣组织。使用免疫组织化学和Western blotting 检测不同组织中的Survivin 和MEF2D 蛋白表达水平，并探讨Survivin 和MEF2D 表达的相关性。结果 Survivin 和MEF2D 在黑色素瘤 组织中阳性表达率高于皮内痣组织（P <0.05）；Survivin、MEF2D 与病理分级有相关性（P <0.05）；Western blotting 检测发现Survivin 和MEF2D 在黑色素瘤组织中的表达高于皮内痣组织（P <0.05）；Survivin 与 MEF2D 蛋白呈正相关（P <0.05）。结论 Survivin 和MEF2D 在黑色素瘤组织中高表达，且与病理分级有相关性， MEF2D 可能通过调节Survivin 表达调控黑色素瘤凋亡，是黑色素瘤发生、发展的重要机制。

Abstract:

Objective To explore the expressions of anti-apoptosis proteins Survivin and MEF2D in melanoma and their correlation. Methods The expressions of Survivin and MEF2D were detected in 21 cases of melanoma tissues and 10 cases of intradermal nevus tissues by immunohistochemistry and Western blot. Then the correlation between Survivin and MEF2D expressions was explored. Results The positive rates of Survivin and MEF2D in the melanoma tissues were significantly higher than those in the intradermal nevus tissues (P < 0.05). The expressions of Survivin and MEF2D were associated with pathological stage (P < 0.05). The expressions of Survivin and MEF2D in melanoma were higher those in the intradermal nevus tissues (P < 0.05). Survivin was positively correlated with the expression of MEF2D (P < 0.05). Conclusions Survivin and MEF2D are highly expressed in melanoma, and correlated with pathological stage. MEF2D might regulate the expression of Survivin to control the apoptosis of melanoma, and it plays a significant role in the development of melanoma.