磷酸化表皮生长因子受体表达与糖尿病皮肤病变关系的实验研究

Effect of phospho- epidermal growth factor receptor on skin lesions of diabetic rat

摘要:

目的??探讨磷酸化表皮生长因子受体（pEGFR）表达与糖尿病皮肤病变的关系。方法??80 只 SD 大 鼠随机分为对照组和模型组，在第 3 天、第 1、2、4、8 周 5 个时间点检测两组皮肤组织中的糖和糖基化终末产 物（AGEs）的含量， 免疫组织化学和 Western?blotting 检测皮肤中 pEGFR 的表达， 并对皮肤进行组织学观察。结果??糖尿病模型复制成功的模型组大鼠毛色暗黄，体重下降 ； 皮肤中糖及 AGEs 含量增加，在第 2、4 和 8 周时与对照组比较差异均有统计学意义（ P ?<0.05） ； 免疫组织化学显示 pEGFR 在皮肤组织的细胞膜和细胞内均有表达，在细胞核中也有少量表达， 可见模型组大鼠在 2 周后染色区域减少且浅染， 随着病程的发展， 浅染区域越来越少， Western?blotting 结果显示皮肤组织中有 pEGFR 的表达， 光密度（OD）值显示其表达下调， 在第 4 和 8 周时与对 照组比较差异有统计学意义（ P ?<0.05） ； 组织切片观察发现在 2 周后表皮变薄，其厚度在第 4 和 8 周时与对照组比较差异有统计学意义（ P ?<0.05） ， 真皮具有萎缩、 炎症细胞浸润现象。结论??糖尿病皮肤中 pEGFR 的表达下降，是导致皮肤病变的重要机制之一。

Abstract:

Objective To investigate the effect of phospho-epidermal growth factor receptor (pEGFR) on skin lesions of diabetic rats. Methods Eighty SD rats were randomly divided into normal control group and diabetic model group. Sugar content and advanced glycation end products (AGEs) of skin tissue were detected at the 3rd day, 1st week, 2nd week, 4th week and 8th week post insults. The expression of pEGFR in the skin was measured by immunohistochemistry and western blotting. Histological change of skin was observed. Result Diabetic rats experienced yellow and dark hair and weight loss. The sugar content and AGEs of the skin tissue was increased significantly in diabetic group at the 2nd, 4th and 8th week post modeling procedure (P < 0.05) when compared with normal control group. Immunohistochemistry results showed expression of pEGFR in cell membranes, cytoplasm and nucleus of skin tissue in diabetic rats. Western blotting suggested decreased expression of pEGFR at the 4th and 8th week in skin tissue of diabetic group when compared with control group (P < 0.05). Morphological analysis witnessed epidermal thinning at 4th and 8th week when compared with the control group (P < 0.05). Diabetic rats also manifested phenomenon of dermal atrophy and inflammatory cells infiltration. Conclusion The decrease of pEGFR expression in diabetic skin is probably involved in skin lesions.