Notch-1、Notch-3 和 Hes-1 在胃癌中的表达及其临床意义

Expression and clinical significance of Notch-1, Notch-3 and Hes-1 in gastric cancer

摘要:

目的??探讨 Notch 信号通路不同受体（Notch-1、Notch-3）及下游基因 Hes - 1 在胃癌中的表达 及与临床病理参数的关系。方法??选取 2014—2016 年中国人民解放军海军军医大学附属长征医院经病理确 诊的具备完整资料的 30 例不同分期的胃癌标本，通过免疫组织化学染色检测 Notch-1、3 及 Hes-1 的表达， 并收集患者临床病理参数进行统计分析。结果??Notch-1、3 及 Hes-1 在胃癌不同分期中均存在表达，其中 Notch-1 和 Hes-1 阳性率在不同的 T 分期中差异有统计学意义（ P <0.05） ，在中晚期患者中较高（ P <0.05） ， 进一步分析表明 Hes-1 和 Notch-1 表达有相关性 （ P <0.05） 。Notch-3 的表达在不同年龄中有差异 （ P <0.05）外，在其他临床病理参数中均无差异。结论??Notch 信号通路中 Notch-1、3 及 Hes-1 在胃癌患者不同临床病理参数中的表达存在一定差异， 未来仍需进一步研究。

Abstract:

Objective To determine the expression of Notch receptors (Notch-1 & 3) and the downstream gene Hes-1 in gastric cancer and potential clinical significance. Methods Thirty gastric cancer patients enrolled in Shanghai Changzheng Hospital during 2014 to 2016 were involved in this study. Clinical-pathological parameters were collected, and expression of Notch-1 & 3 and Hes-1 were measured by immunohistochemical staining. Results Notch-1 & 3 and Hes-1 were heterogeneously expressed in the patients with different stage while patients with advanced stage of tumor experienced increased levels of Notch-1 and Hes-1 (P < 0.05). Expression of Hes- 1 was positively correlated with Notch-1 (P < 0.05). However, expression of Notch-3 was not affect by all clinical parameters except ages (P < 0.05). Conclusions Expression of Notch-1 & 3 and Hes-1 is likely to be affected in gastric cancer patients, and additional studies are necessary.