水通道蛋白 1、血管内皮生长因子及微血管密度 在胃癌组织中的表达及临床意义

Expression and clinical significance of aquaporin-1, vascular endothelial growth factorand and microvessel density in gastric carcinoma

摘要:

目的??探讨水通道蛋白 1（AQP1） 、血管内皮生长因子（VEGF） 、微血管密度（MVD）在胃癌组织中 的表达及 AQP1、VEGF、MVD 之间的相关性和临床意义。方法??选取郑州大学第二附属医院胃癌标本 79 例。每 例分别取癌组织和癌旁组织（距肿瘤边缘 <2?cm 处） ，采用免疫组织化学方法检测 AQP1、VEGF 与 MVD 在人 胃癌组织和癌旁组织中的表达，对 AQP1、VEGF 与 MVD 的表达及其与临床病理参数的关系进行分析。结果? AQP1、VEGF 在胃癌组织与癌旁组织比较，差异有统计学意义（ P ?<0.05） ，胃癌组织高于癌旁组织 ； MVD 在 胃癌组织的表达与癌旁组织比较，差异有统计学意义（ P ?<0.05） ，胃癌组织高于癌旁组织 ； AQP1、VEGF 及 MVD 与胃癌的分化、淋巴结转移、脉管癌栓及分期相关（ P ?<0.05） ； 胃癌组织中 AQP1 与 VEGF 的表达呈正 相关（ r =0.497， P ?<0.05） ； 在 VEGF 阳性表达的胃癌组织中， MVD 表达高于 VEGF 阴性表达的组织（ P ?<0.05） ，在 AQP1 阳性表达的胃癌组织中，MVD 表达高于 AQP1 阴性表达的组织（ P ?<0.05） 。结论??AQP1、VEGF、MVD 存在协同作用， 与肿瘤的发生、发展密切相关。

Abstract:

Objective To investigate the expression of aquaporin-1 (AQP1), vascular endothelial growth factor (VEGF) and microvessel density (MVD) in gastric carcinoma and their clinical significance. Methods Totally 79 gastric cancer patients who were admitted into our hospital from September 1, 2015 to June 1, 2017 were involved in this study. Tumor specimens and adjacent normal tissues (> 2 cm away from the tumor) were collected. Expression of AQP1, VEGF and MVD were identified by immunohistochemistry. Correlation among AQP1, VEGF, MVD and the clinicopathological parameters were determined. Results The expression of AQP1, VEGF and MVD in gastric carcinoma tissue was increased significantly when compared with those in para-cancerous tissue (P < 0.05). AQP1, VEGF and MVD were closely correlated with tumor differentiation, lymph node metastasis, vascular tumor thrombosis and staging (P < 0.05). Spearman correlation analysis showed that AQP1 was positively associated with VEGF expression (r = 0.497, P < 0.05). MVD was enhanced in VEGF positive and AQP1 positive cancer tissues compared with that in VEGF negative or AQP1 negative tissue (P < 0.05). Conclusions