TGF-β 1 和 β-catenin 在儿童结肠息肉中的表达及意义

Expression and clinical significance of TGF-β 1 and β-catenin in colon polyps of children

摘要:

目的??分析转化生长因子 β 1 （TGF-β 1 ）和 β- 连环蛋白（β-catenin）在儿童结肠息肉中的表达 及临床意义。方法??采用免疫组织化学法检测 TGF-β 1 和 β-catenin 蛋白在儿童结肠息肉中的表达情况，逆转 录聚合酶链反应（RT-PCR）检测 TGF-β 1 和 β-catenin?mRNA 的表达水平，分析其相关性。结果 TGF-β 1 蛋白在儿童单发和多发幼年性息肉组织中的阳性表达率为 27.59% 和 48.28%，β-catenin 蛋白阳性表达率分别 为 13.79% 和 41.38%，TGF-β 1 和 β-catenin 的表达呈正相关（ r =0.463， P =0.000） 。多发幼年性息肉组织中TGF-β 1 和 β-catenin?mRNA 相对表达水平高于正常组，差异有统计学意义（ P <0.05） 。结论 TGF-β 1 和β-catenin 可能参与了儿童结肠息肉的发生和进展。

Abstract:

Objective To analyze expression of TGF-β 1 and β-catenin in colon polyps of children and potential clinical significance. Methods Expression of TGF-β 1 and β-catenin protein in colon polyps of children was detected by immunohistochemistry and RT-PCR. Potential correlations between TGF-β 1 and β-catenin were analyzed. Results The positive expression rates of TGF-β 1 protein in children with single and multiple juvenile polyps were 27.59% and 48.28%, respectively. Positive expression rates of β-catenin in children with single and multiple juvenile polyps were 13.79% and 41.38%, respectively. TGF-β 1 was positively correlated with β-catenin (r = 0.634, P < 0.01). Expression levels of TGF-β 1 and β-catenin in multiple juvenile polyp tissue were significantly higher than those in the normal tissue (P < 0.05). Conclusions TGF-β 1 and β-catenin are involved in the development and progression of colon polyps in children.